Separator for CD8 and CD4 Isolation

Johns Hopkins School of Medicine
Oncology
Lab Technician

Overall

Performance

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

QuadroMACS Separator

Catalog Number:

130-090-976

Our lab wants a clean CD8+ or CD4+ population that we can use for in-vitro assays. Therefore, we use this magnet for our isolations. It is sturdy and efficient.

Experimental Design and Results Summary

Application

Isolation of specific T cells

Protocol Overview

After incubation per manufacturer protocol for the isolation kits, we would elude the samples with a column that attaches to the magnet of the separator. The samples negatively selected for will go through the column. The positive selection can be obtained with a plunge of the column.

Tips

The tube rack that comes with the magnet does fall apart easily.

Features Summary

Depending on your isolation kit, the specific population that you want can easily be separated with this magnet.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

A very sturdy and efficient separator

The Bad

The magnet can be heavy and therefore difficult to carry.

The Bottom Line

A very sturdy and effective way of separating the population of your choice

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