Very Strong Secondary Antibody for Western Blot

November 05, 2018

Oncology
Johns Hopkins School of Medicine
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell Signaling Technology

Product Name:

Anti-rabbit IgG HRP-linked Antibody

Catalog Number:

7074

Our lab is interested in T cells and tumor cells in relation to metabolism. We use this secondary antibody for western blot after incubation with the primary overnight.

Experimental Design and Results Summary

Applications

Western Blot

Sample

T cells

Primary Incubation

1:5000 in Room Temperature for 1.5 hrs

Blocking Agent

5% nonfat dry milk

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

ECL Invitrogen

Results Summary

We have been using this secondary antibody for years and it has always been consistent. CST suggests we use 1:2000 for dilution; however, we have found that 1:5000 works best for situations where we would need to re-probe the next day.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very robust and consistent antibody for secondary in western blot

The Bad

None

The Bottom Line

Definitely worth the investment. It is always reliable and I've never had any problem with not being able to see signal.

Share your experience with other scientists. Write a Review! »

Join the discussion