Great Antibody For B Cell Activation

August 21, 2017

Molecular and Cell Biology
University of California, Merced
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Jackson ImmunoResearch Laboratories, Inc.

Product Name:

AffiniPure F(ab')₂ Fragment Goat Anti-Mouse IgM, µ Chain Specific (min X Hu,Bov,Hrs Sr Prot)

Catalog Number:

115-006-075

We aim to understand the influence of T cells on antibody-mediated disease. In general we use this antibody to stimulate B cells ex vivo for cell culture assays. The concentration at which we use it at can vary depending on desired results but this antibody allows us to stimulate B cells with ease for a range of culture experiments.

Experimental Design and Results Summary

Applications

Cell Stimulation

Sample

Murine B cells

Primary Incubation

5ug/ml in culture with complete media for up to 6 days.

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

None

Results Summary

We analyze B cells at time points between 36 hours for activation and 4 days for antibody production after stimulation with anti-IgM. At the concentration we use this antibody at B cells have increased surface MHC at 36 hours and proliferate to maintain live cell numbers for up to 4 days. At 6 days we can observe an increase in antibody production.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

It works well at a variety of concentrations to stimulate B cells.

The Bad

It may not be as effective after 3 months of storage at the recommended 4 degrees.

The Bottom Line

It will work well but may require some optimization. The attempts to optimize are well worth the cost and efficacy of this product.

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