Good General CD8 Antibody

August 09, 2017

Molecular and Cell Biology
University of California, Merced
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen Antibodies

Product Name:

CD8a Monoclonal Antibody (53-6.7), APC-eFluor 780, eBioscience™

Catalog Number:

47-0081-82

We routinely use this antibody and this clone in varying fluorophores, for general CD8 T cell staining during autoimmune disease. We have used this product in a variety of experiments from surface staining to cell stimulations and cell sorting. In all cases this product presents as a clear separate populations. However, there are some fluorophores that may give brighter staining than this particular color.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Lymphocytes and Splenocytes

Primary Incubation

Dilute 1:300 for 30 minutes at 4 degrees Celcius

Blocking Agent

FC Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR II or BD FACS Aria II

Results Summary

We have seen clear and distinct populations using this particular clone and fluorophore combination. The staining is not as bright as other colors on this clone have been shown to be. However, this conjugate remained stable even when running fixed samples 4 days after staining. The compensation between this color and APC and be challenging.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Consistent and clear staining results in a variety of applications

The Bad

Doesn't titrate as well as other color fluorophore even in the same clone.

The Bottom Line

This is a good antibody if you need to stain for CD8a in this color. There may certainly be better options but this one works consistently well for general use.

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