Invitrogen Antibodies
CD4 Monoclonal Antibody (RM4-5), PerCP-Cyanine5.5, eBioscience™
45-0042-82
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We use this antibody in a number of staining panels to investigate T cell responses during autoimmune disease. We have used this antibody for direct surface staining, with fixation in conjugation with transcription factor staining, after stimulation and for routine T cell sorting. In all applications this antibody is bright and reliable and can be diluted fantastically.
Flow Cytometry
Murine Lymphocytes and Splenocytes
Dilute 1:1600 for 30 minutes at 4 degrees Celcius
FC Block
None
BD LSR II or BD FACS Aria II
Even at a 1:1600 dilution for 5 million cells we always see bright staining of CD4 with PerCP-Cyanine5.5. We have not experienced any color breakdown even after running fixed cells 4 day after staining. As with other CD4 antibodies, cell stimulation can down-regulate the CD4 protein on the cell surface making the population appear less distinct.
N/A
Dilutes fantastically with stable flourophore color.
Compensation of PerCP-Cyanine5.5 can be challenging with PE, PeCy7 and TexasRed.
All together an excellent antibody for general purpose CD4 staining.