BD Biosciences
BV421 Rat Anti-Mouse CD326
563214
Info:Supplier Page View Product SpecsView Product
Browse Similar Products: CD326 Flow Cytometry Antibodies
Usually when we do flow experiments, we are looking to isolate whole epithelial populations and sub-populations. Epcam is a great marker for this, so we use it to positively select for our cells. We typically use this in whole mouse lung that has been prepared as according to our standard flow prep protocol.
Flow Cytometry
Single cell suspension of processed whole mouse lung
30 minutes at 1:250; 10 million cells per ml
Fc block for ~10-15 minutes
None
Flow cytometer
This Epcam antibody produces clean and reliable staining in mouse lungs. It accurately marks epithelial cells and stains very clearly, making it easy to gate!
N/A
Reliable & clean
Nothing!
Clear, reliable, Epcam staining as long as you do an Fc receptor blocking step beforehand!