CD132 (Gamma Chain Subunit) Staining Of B Cells

Immunology
Northwestern University
Grad Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

APC anti-mouse CD132

Catalog Number:

132307

The aim of the research was to study germinal center B cells. The goal for the experiment was to measure the levels of different receptors and compare them between non-germinal center and germinal center B cells from immunized mice. I chose this product because of the price and it fight my flow cytometry panel.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1:30, 4 degrees, 1 hour

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Clearly stain B cells expressing gammaC

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use, clearly marks positive cells

The Bad

None

The Bottom Line

Great antibody for measuring gamma chain expression on cells by flow cytometry.

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