Thermo Scientific
Pierce Streptavidin Magnetic Beads
88816
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The general aim was to use streptavidin coated magnetic beads in order to capture biotin labeled protein. The goal was to purify biotin-labeled palmitoylated proteins from whole cell lysates. This product was selected due to the ease of capture utilizing the magnetic properties of the beads in conjunction with the DynaMag-2 magnetic particle collector.
Protein purification
Rat hepatoma whole cell lysates
Palmitoylated proteins were labeled with a biotin containing analog. Lysates were incubated with Pierce Streptavidin magnetic beads for 1 hour at room temp (55ug protein: 1mg bead). Proteins were eluted using reducing agents, DTT, BME or 0.1M glycine pH 2.0.
Perform a dose response protein:bead ratio
The beads work well. The problem we are running into is that it is difficult to quantify the protein concentration after eluting sample from the bead. The elution techniques suggested require harsh reducing agents, or low pH conditions, which make running a BCA tricky.
N/A
The magnetic properties allow rinsing, and capturing each phase of the protocol very easy!
Difficult to quantify protein concentration due to harsh elution suggestions
Easy to use, but requires a bit of optimization.
Product Name: Pierce Streptavidin Magnetic BeadsSupplier Page