Vector Laboratories
VECTASHIELD Antifade Mounting Medium with DAPI
H-1200
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We take frozen sections of mouse whole eyes to examine the different layers of the eye. This vectashield allow us to protect our stained cells on these sections and also adds a DAPI layer.
Mount frozen cryo-sections onto slide and stain for DAPI
Frozen sections of mouse whole eyes
Frozen sections are fixed in 4% paraformaldehyde for 30 minutes and then washed in cold PBS 3 times. Vectashield is dropped sparingly onto the slides and coverslip is added gently.
Don't use too much or it will squeeze out.
This medium will not fade even over months (if slides are stored in the dark) and clearly stains Dapi in the frozen sections.
N/A
Protects slides from fading
None
Great for staining frozen cryo-sections with DAPI and protecting the other stains from fading over time.
Product Name: VECTASHIELD® with DAPISupplier Page