Thermo Fisher Scientific
Isolectin GS-IB4 From Griffonia simplicifolia, Alexa Fluor® 488 Conjugate
I21411
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Our lab studied abnormal vessel growth in mouse retinas and this antibody is crucial for staining and imaging these vessels ex vivo.
Immunofluorescence
Fixed whole mouse retina
1:200 primary antibody, 2 hours at room temperature in staining buffer (or overnight at 4 degrees celcius)
Goat serum
None
Fluorescent microscope
We are able to clearly stain for small fixed vessels in the mouse retina with little fluorescent background signal and no non-specific staining.
N/A
Very bright signal, little background, fast stain
This is a great antibody for staining small vessels ex vivo.