Excellent CD69 Antibody

Immunology
Columbia University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Anti-human CD69 BV421

Catalog Number:

310929

I have used many CD69 antibodies and this is one of the best. For a marker like CD69 that exists at a variety of expression levels a super bright fluorochrome like bv421 is great. This product is super easy to use and retains signal after fix perm as well.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human splenocytes

Primary Incubation

30 min on ice

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSRII

Results Summary

Image shows separation. Cells already gated on FSC/SSC, CD3+

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Great separation

The Bad

Expensive

The Bottom Line

One of the best CD69 antibodies

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