Biolegend
APC Anti-human CD3
300312
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Browse Similar Products: CD3 Flow Cytometry Antibodies
This APC CD3 antibody from biolegend works quite well. Works best at 1:200 or even 1:400. Even at 1:800 get good separation. Withstands fix/perm for intracellular staining just fine. However, you probably don't need such a bright color like APC for an abundant marker like CD3.
Flow Cytometry
Human PBMCs
25 min, 4C, dark
Staining done in PBS + 1% FBS + azide
None
BD Fortessa
See image. This is already gated on FSC and SSC.
Very bright, easy to use
Great antibody for detection of CD3. If you are using a panel with many colors htough, may want to save bright colors like APC for antigens that have lower expression