Biolegend
APC-Cy7 Anti-mouse CD4
100526
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CD4, also known as L3T4 or T4 is expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II. The clone RM4-5 is a well known anti-mouse CD4 antibody clone and blocks the binding of GK1.4 antibody, which is a known depleting agent of CD4 T cells.
Flow Cytometry
Mouse splenocytes
25 minutes on ice
CD16/32 antibody for blocking FC receptors
None
Flow cytometry on APC-Cy7 channel
The staining was relevant; however, as usual, the Cy7 modified fluorophores are known to be very bright and bleed significantly into adjacent channels, complicating the compensation parameters. A small level of background shift was also seen in the negative population.
Bright and relevant staining
Background levels are noticeable
Good antibody for using as phenotyping marker or compensation control but probably not for measuring expression kinetics.