eBioscience
PE anti-mouse CD4
12-0042-83
Info: View Product View Product Specs
Browse Similar Products: CD4 Flow Cytometry Antibodies
PE or phyco-erythrin is my favorite fluorophore and I used it for detecting CD4 T cells within mouse splenocytes. Thus far, this was the best staining I have ever seen. The fluorescence shift was optimal, no background or bleeding and the populations were relevant. A highly recommended antibody; perhaps a highly recommended fluorophore as I have noticed it to work beautifully with pretty much any target.
Flow Cytometry
Mouse splenocytes
25 minutes on ice
CD16/32 antibody for blocking Fc receptors
None
Flow cytometry on PE channel
The staining worked well with about 2 log increase in fluorescence of positive cells. The background was minimal and frequencies of positive and negative cells were physiologically relevant.
Perfect staining
None that I can think of
PE is the most desired fluorophore. If you have it available in your panel, then use it for any target.