eBioscience
PE-Cy7 anti-mouse CD4
25-0042-82
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This antibody was used as a compensation control for the PE-Cy7 channel. Although it worked it just fine for my intended purpose, there are a few issues I noticed with the staining. First, even at 1:500 dilution the fluorescence was very bright (>3 log shift) which is perhaps an attribute of the PECy7 dye. Second, the negative population showed some background staining which is an issue for experiments that look at changes in MFI values.
Flow Cytometry
Mouse splenocytes
25 minutes on ice
CD16/32 antibody for blocking FC receptors
None
Flow cytometry on PE-Cy7 channel
The CD4 positive cells were stained well and showed relevant expression. The negative fraction of cells have some background staining.
Very bright fluorescence
Background staining on negative cells
A good product for measuring CD4+ cells, but not for measuring MFI changes.