hTIM-3 Antibody Review

Neurosurgery
BWH/HMS
postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC/Cy7 anti-human CD366 (Tim-3) Antibody

Catalog Number:

345025

This antibody was used to stain human PBMC cell lines after OK3 treatment. This is a good, but not excellent antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

30min

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

APC-Cy7

Results Summary

hPBMCs were stimulated 3 days with anti-CD3.cells were washed with Washing buffer 2 times, incubated with the antibody for 30 min at RT (in a final volume of 10ul) and then washed again 2 times. Samples were read at the LRSII.

Additional Notes

None

Related Categories

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Summary

The Good

Easy to use

The Bad

Not a strong signal.

The Bottom Line

Good antibody - maybe it is not the best around.

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