ATM Antibody Review

Microbiology
Medical College of Wisconsin
Research Technologist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Cell Signaling Technology

Product Name:

ATM Antibody

Catalog Number:

2873S

This antibody was used for assessing ATM levels in bone marrow derived macrophages in virus infected mice.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Murine macrophages

Primary Incubation

1 hour, RT

Blocking Agent

2% Egg Albumin

Secondary Incubation

Overnight, 1:200, 4C

Tertiary Incubation

45 Minutes, 1:20,000 Rabbit

Detection

Developer

Results Summary

The transfer for this antibody is tricky, it takes a long time at the right voltage because it is a large protein. Once optimized the antibody worked great, I think it was just time consuming because of the nature of the protein.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Robust

The Bad

Hard to optimize

The Bottom Line

Would recommend this antibody for ATM.

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