Abcam
PKA Kinase Activity Assay Kit (ab139435)
ab139435
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I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's systems using anti-mycobacterial ESAT6 binding peptides.
Studying PKA activation
Cell lysates
IBMX pretreated cells were stimulated with mycobacterial antigens in presence or absence of cAMP analog, after 60 minutes, cells were washed, lysed and tested on PKA activity assay.
Use of IBMX or any other PDE inhibitor is important
Increased PKA activity was observed in presence of mycobacterial antigen which was further enhanced with use of cAMP analog.
None
Non-radioactive
Good non-radioactive PKA activity assay kit.
Product Name: PKA Kinase Activity Assay KitSupplier Page