PKA Kinase Activity Assay Kit ab139435 from Abcam

Product Specs
ItemPKA Kinase Activity Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab139435
Quantity1 96TESTS
Sample TypeAdherent cells, Suspension cells, Tissue Extracts, Purified protein
Assay Time4h 30m
ApplicationsCellular Assay
TypeEnzyme activity
Detection TargetProtein kinase A

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Website: http://www.abcam.com

(1) Fibroblast growth factor 5 protects against spinal cord injury through activating AMPK pathwayJournal of Cellular and Molecular MedicineNovember 10, 2023Feng Chen, Bing‐Rui Xiong, Shu‐Yue Xian, Jing Zhang, Rui‐Wen Ding, Ming Xu, Zong‐Ze Zhangwere detected using a competitive cAMP Assay Kit (#ab65355; Abcam) and PKA Kinase Activity Assay Kit (#ab139435; Abcam) as previously described. 36 2.9 Statistical analysis Data are analysed by SPSS software (version

(2) Sublethal biochemical, behavioral, and physiological toxicity of extremely low dose of bendiocarb insecticide in Periplaneta americana (Blattodea: Blattidae)Environmental Science and Pollution ResearchFebruary 6, 2023Milena Jankowska, Bartosz Augustyn, Justyna Maliszewska, Barbara Przeździecka, Dawid Kubiak, Olga Chełchowska, Jagoda Kaczorek, Dominik Knop, Kornelia Krajnik, Hanna Kletkiewicz, et al.perform direct immunoassays accordingly to the producer’s instruction: ab139437 for PKC activity and ab139435 for PKA activity (Abcam). Protein kinase level was measured colorimetrically on plate reader (BioTek,

(3) Alkaline Dilution Alters Sperm Motility in Dairy Goat by Affecting sAC/cAMP/PKA Pathway ActivityInternational Journal of Molecular SciencesJanuary 16, 2023Qifu He, Feng Gao, Shenghui Wu, Shaowen Wang, Zhiming Xu, Xuerui Xu, Tianyang Lan, Kang Zhang, Fusheng Quanto top read mode at Ex/Em = 540/590 nm. For PKA evaluation, we used a PKA Kinase Activity Assay Kit (ab139435, Abcam, Cambridge, United Kingdom) according to the manufacturer’s instructions. Finally, 20 µL of stop

(4) Melatonin influences the biological characteristics of keloid fibroblasts through the Erk and Smad signalling pathwaysBurns & traumaJanuary 1, 2023Huang S, Deng W, Dong Y, Hu Z, Zhang Y, Wang P, Cao X, Chen M, Cheng P, Xu H, et al.assay PKA activity in the cell lysates was assayed according to the manufacturer’s protocol (Abcam; ab139435). Six replicates were made for each group. The activity of PKA in the samples was then determined by

(5) Cancer cell-derived exosomal miR-425-3p induces white adipocyte atrophyAdipocyteDecember 31, 2022Anwen Liu, Wenxia Pan, Shutong Zhuang, Yuanzhi Tang, Haitao ZhangCambridge, MA, USA), cAMP Enzyme Immunoassay Kit (CA200, Sigma-Aldrich), and PKA Kinase Activity Assay Kit (ab139435, Abcam) were used to measure cell proliferation, glycerol content in culture medium, intracellular cAMP

(6) Loss of SUMO-specific protease 2 causes isolated glucocorticoid deficiency by blocking adrenal cortex zonal transdifferentiation in miceNature communicationsDecember 21, 2022Dufour D, Dumontet T, Sahut-Barnola I, Carusi A, Onzon M, Pussard E, Wilmouth JJ, Olabe J, Lucas C, Levasseur A, et al.total, 10 µg of protein from 4-week-old adrenals were used for measurement with PKA kinase activity kit (ab139435, Abcam). In vitro SUMOylation assay Protein purification (SUMO2, SAE1/UBA2, UBC9) and in vitro SUMOylation

(7) A remarkable adaptive paradigm of heart performance and protection emerges in response to marked cardiac-specific overexpression of ADCY8eLifeDecember 14, 2022Tarasov KV, Chakir K, Riordon DR, Lyashkov AE, Ahmet I, Perino MG, Silvester AJ, Zhang J, Wang M, Lukyanenko YO, et al.were performed for cAMP-dependent protein kinase (PKA) using the PKA Kinase Activity Assay Kit (Abcam, ab139435), in accordance with the manufacturer’s instructions. The units are expressed in OD/mg protein/min. Western blotting

(8) Heat Shock Protein 70 Mediates the Protective Effect of Naringenin on High-Glucose-Induced Alterations of Endothelial FunctionInternational Journal of EndocrinologyAugust 1, 2022Zhihan Zhang, Hui Liu, Xiang Hu, Yikang He, Lu Li, Xinyu Yang, Changhua Wang, Mingbai Hu, Shengxiang TaoPKA Activity Glucose Uptake Assay Kit (Colorimetric, ab136955, Abcam) and PKA Kinase Activity Kit (ab139435, Abcam) were used to measure glucose uptake and PKA activity in HUVECs, respectively, according to the

(9) Impaired oxygen-sensitive regulation of mitochondrial biogenesis within the von Hippel-Lindau syndromeNature metabolismJune 1, 2022Li S, Li W, Yuan J, Bullova P, Wu J, Zhang X, Liu Y, Plescher M, Rodriguez J, Bedoya-Reina OC, et al.temperature. Subsequently, TFAM phosphorylation by PKA was assayed using PKA Kinase Activity Assay Kit (ab139435, Abcam) according to the manufacturer’s instructions. Samples were centrifuged at 8,000g for 30 s and

(10) mTORC1 induces plasma membrane depolarization and promotes preosteoblast senescence by regulating the sodium channel Scn1aBone researchMarch 8, 2022Chen A, Jin J, Cheng S, Liu Z, Yang C, Chen Q, Liang W, Li K, Kang D, Ouyang Z, et al.and mitochondrial PKA activities were measured with a nonradioactive PKA Kinase Activity Assay Kit (#ab139435, Abcam) according to the manufacturer’s instructions.59 Statistical analysis All results are presented

(11) Phosphodiesterase 4D promotes angiotensin II-induced hypertension in mice via smooth muscle cell contractionCommunications BiologyJanuary 20, 2022Tianfei Fan, Yangfeng Hou, Weipeng Ge, Tianhui Fan, Xiaohang Feng, Wenjun Guo, Xiaomin Song, Ran Gao, Jing Wangactivity in aortic tissues or RASMCs was detected via a PKA kinase activity assay kit (Abcam, Cat#: ab139435) according to the manufacturer’s instructions. Absorbance was measured at OD = 450 nm via a multi-mode

(12) Blood-Brain Barrier Permeability: Is 5-Hydroxytryptamine Receptor Type 4 a Game Changer?PharmaceuticsNovember 3, 2021Becker G, Da Silva S, Sabo AN, Antal MC, Kemmel V, Monassier L.3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay (#ab197010), the Protein Kinase A (PKA) activity kit (#ab139435) and phosphorylated Src and total Src kit (#ab207461) were obtained from Abcam. 2.2. Cell Culture Human

(13) Enhanced Heart Failure in Redox‐Dead Cys17Ser PKARIα Knock‐In MiceJournal of the American Heart AssociationOctober 5, 2021M. M. Towhidul Islam et al.samples, a cAMP ELISA kit (Enzo Life Sciences, Lausen, Switzerland) and a PKA kinase activity assay (ab139435; Abcam, Berlin, Germany) were performed according to the manufacturer´s protocol. Histology Cardiac

(14) Overexpressing miR-122-5p Inhibits the Relaxation of Vaginal Smooth Muscle in Female Sexual Arousal Disorder by Targeting Vasoactive Intestinal Peptide Receptor 1Sexual MedicineAugust 1, 2021Shengnan Cong, Tao Gui, Qinchuan Shi, Jingjing Zhang, Jingyi Feng, Lianjun Pan, Jiehua Ma, Aixia ZhangGermany). PKA Kinase Activity Assay The activities of PKA were examined with a PKA enzyme activity kit (ab139435, Abcam, Cambridge, UK). Briefly, 1 × 106 cells were collected and washed twice in ice-cold phosphate-buffered

(15) miR‑351‑5p aggravates lipopolysaccharide‑induced acute lung injury via inhibiting AMPKMolecular Medicine ReportsJuly 30, 2021Fen Hu, Xianfeng Dong, Weixin Li, Jianfa Lv, Feng Lin, Gan Song, Guoqiang Hou, Ruiyun Liab216165), cAMP assay kit (cat. no. ab65355) and protein kinase A (PKA) activity assay kit (cat. no. ab139435) were obtained from Abcam. Compound C (CC; cat. no. S7840), a selective AMPK inhibitor, was purchased

(16) Cardiac Fibroblasts Mediate a Sexually Dimorphic Fibrotic Response to β-Adrenergic StimulationJournal of the American Heart AssociationJune 1, 2021Peter AK, Walker CJ, Ceccato T, Trexler CL, Ozeroff CD, Lugo KR, Perry AR, Anseth KS, Leinwand LA.quantify protein concentrations in the lysed samples. PKA activity was determined using assay kit (Abcam ab139435). Relative PKA activity was calculated by normalizing PKA activity to total protein concentration. Table. Primers

(17) AKAP1 Deficiency Attenuates Diet‐Induced Obesity and Insulin Resistance by Promoting Fatty Acid Oxidation and Thermogenesis in Brown AdipocytesAdvanced ScienceMarch 1, 2021Lele Ji et al.and mitochondrial PKA activities were measured by the nonradioactive PKA Kinase Activity Assay Kit (ab139435, Abcam) following manufacturers' instructions. Assessment of Oxygen Consumption Rate Oxygen consumption

(18) Cichoric Acid Ameliorates Monosodium Urate-Induced Inflammatory Response by Reducing NLRP3 Inflammasome Activation via Inhibition of NF-kB Signaling PathwayEvidence-Based Complementary and Alternative MedicineJanuary 6, 2021Qi Wang, Bingfeng Lin, Zhifeng Li, Jie Su, Yulin FengAlternative Medicine anti-TNF-α antibody (ab269282), anti-NLRP3 antibody (ab263899), PKA kinase activity assay kit (ab139435), Prostaglandin E2 ELISA Kit (ab133021), and cAMP assay kit (Competitive ELISA) (ab234585) (Abcam, Cambridge,

(19) MicroRNA-137-3p Improves Nonalcoholic Fatty Liver Disease through Activating AMPKαAnalytical cellular pathology (Amsterdam)January 1, 2021Yu Y, He C, Tan S, Huang M, Guo Y, Li M, Zhang Q.nm. Hepatic protein kinase A (PKA) activity was detected using the PKA Kinase Activity Assay kit (#ab139435; Abcam) based on a synthetic PKA-specific substrate. For the determination of adenyl cyclase (AC) activity,

(20) Isoquercetin Improves Inflammatory Response in Rats Following Ischemic StrokeFrontiers in neuroscienceJanuary 1, 2021Shi Y, Chen X, Liu J, Fan X, Jin Y, Gu J, Liang J, Liang X, Wang C.(TdT)-mediated dUTP-biotin nick end labeling (TUNEL) assay. Protein kinase A (PKA) kinase activity assay kit (ab139435) was purchased from Abcam Ltd. Tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and IL-6 enzyme-linked immunosorbent assay

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3/15/2016PKA Kinase Activity Assay Kit Sachin SamuchiwalI studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice system using anti-mycobacterial ESAT6 binding peptides.

PKA Kinase Activity Assay Kit from Abcam

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PKA Kinase Activity Assay Kit from Abcam

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