HDAC-2 Antibody Review

Microbiology
Medical College of Wisconsin
Research Technologist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma-Aldrich

Product Name:

HDAC-2 Antibody

Catalog Number:

H3159

Browse Similar Products: HDAC-2 Western Blot Antibodies

Infected or non-infected primary murine macrophages are western blotted for various proteins.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Primary Macrophages

Primary Incubation

5% Milk 1 hour

Blocking Agent

Milk in TBST

Secondary Incubation

1:1,000 Overnight at 4C

Tertiary Incubation

1:20,000 at RT for 45 Minutes

Detection

Developer

Results Summary

Optimization of this antibody proved difficult, although that could be intrinsic to our samples. This is a good antibody and can be reused up to 3 times reliably.

Additional Notes

None

Related Categories

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Summary

The Good

Keeps well, reusable

The Bad

Optimization difficult

The Bottom Line

Would recommend this antibody for HDAC-2 western blotting.

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