cDNA Synthesis Kit

Miami
MI
Student

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Company:

Applied Biosystems

Product Name:

High Capacity cDNA Reverse Transcription Kit

Catalog Number:

4368813

This kit is used to transform RNA into cDNA. This kit contains a random primer mix and the RT is not already into the buffer. This means one can have true - RT controls. The kit also contains RNase inhibitor to minimize RNA degradation from RNAse.

Experimental Design and Results Summary

Application

cDNA synthesis

Starting Material

None

Protocol Overview

Mix kit components in a master mix. Add master mix to RNA and add appropriate volume of nuclease free water.

Tips

Mix everything well before making the master mix

Results Summary

This kit has been used to make cDNA from RNA. The cDNA work well in downstream applications.

Additional Notes

None

Image Gallery

Summary

The Good

RNase Inhibitor

The Bad

Only random primers. No poly-A.

The Bottom Line

This is a good kit to make cDNA.

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