Santa Cruz Biotechnology
ASCL-4
SC-134507
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ASCL-4 was used to test the purity of a subcellular fractionation experiment. This particular antibody was used to monitor the purity of the mitochondrial associated membrane fraction.
Western Blot
Rat liver
1:2000 4C overnight with rocking
1:3 Odyssey block:TBST (0.1% tween)
1:15,000 Goat-anti-rabbit (Licor IRDye800CW goat-anti-rabbit IgG), 1 hour, room temp
NA
Odyssey imaging
Really nice, clean results with minimal background. I found that using a 1:1000 dilution produced a non-specific band near the MW of ASCL-4 (75Kda). However, a 1:2000 dilution cleared it up really well and I am happy with the results so far. Santa Cruz sends a decent quantity of antibody, this should get me through quite a few westerns.
None
Clean, crisp image. Not a ton of non-specific background.
I haven't identified any drawbacks yet
Great antibody, works great for identifying organelles!