CD8 FITC Antibody Review

October 26, 2015

Microbiology
Medical College of Wisconsin
Research Technologist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

CD8 FITC Antibody

Catalog Number:

100705

Info: View Product View Product Specs

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Mice are infected with the virus until it's latent, then splenocytes or PEC's are collected, RBC lysed, blocked, and stained.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

1:100, 15 minutes

Blocking Agent

Fc Block

Secondary Incubation

1:400, 15 minutes

Tertiary Incubation

NA

Detection

Flow Cytometry LSRII

Results Summary

Splenocytes and PECs both stain reliably for CD8+ cells using this antibody.

Additional Notes

None

Related Categories

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Summary

The Good

Reliable

The Bad

Relatively robust but at lower dilutions or longer incubation times

The Bottom Line

Good antibody, would recommend for anyone needing to identify CD8+ cell populations.

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