Good Alternate Fluorochrome for the AmCyan Channel

Research
Bloodworks Northwest
Postdoctoral research fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BV510 Hamster Anti-Mouse TCR β Chain

Catalog Number:

563221

Browse Similar Products: TCR beta Chain Antibodies

I tried the anti-TCRbeta (clone H57) antibody conjugated to Brilliant Violet 510 when designing a multicolor panel to identify specific T cell subsets and their expression of activation markers. This fluorochrome works great in the AmCyan channel.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

1:100, 30 mins at +4c.

Blocking Agent

Fc-block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

LSR II

Results Summary

Good staining and separation between positive and negative populations. Voltage adjustments might be necessary to minimize spill over between this fluorochrome and the PacBlue/EF450/BV421 channel.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Great fluorochrome

The Bad

None

The Bottom Line

Great option if you're looking to utilize the AmCyan Channel.

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