Analytik Jena
qTOWER
844-00502-3
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We are interested in minimum residual disease also called MRD. We used the qTOWER for our studies on MRD and we gained 2 points for Ct values.
MRD study
Bone Marrow infiltrated with tumor cells
RNA stabilisation and extraction efficiency using the PAXgene blood RNA tubes. RNA is extracted from PB, BM stabilised in PAXgene blood RNA tubes using the PAXgene blood RNA kit according to the manufacturer’s instructions with a DNAse digestion step before elution of RNA from the column. RNA samples (1 µg) were subjected to reverse transcription using Reverse Transcriptase (Roche) according to the supplier’s instructions. cDNA products were amplified on a qTOWER in 20 µl of reaction mixture containing the SYBR Green I Master (Roche) and 50 mM of forward and reverse primers Each amplification cycle consisted of an initial step at 95°C (5 minutes), followed by 45 cycles of denaturation at 95°C (10 seconds), annealing at 60°C (10 seconds), and extension at 72°C (30 seconds).
None
We tested the performence of the qTOWER by dilution of tumor cells in BM. We gain 2 points for Ct values and we were able to detect 1 tumor cell in 10^6 BM cells. Image shows intensity/cycles, all experiments were done in triplicate and each color is another dilution.
Performance
Program not intuitive enough.
Very good product, can be adapted to any purpose.
Product Name: qTOWER³ Real-Time PCR Thermal Cycler from Analytik JenaSupplier Page