qTOWER³ Real-Time PCR Thermal Cycler from Analytik Jena

Product Specs
ItemqTOWER³ Real-Time PCR Thermal Cycler
CompanyAnalytik Jena
Price Get Pricing
Catalog Number844-0050x-x
Excitation Source4 high-performance long-life RGBW-LED
Temperature Range4°C to 99°C
Dimensions(WxDxH) 275 x 275 x 585 mm
QuantityEA
DetectionUp to 6 different color modules; CPM (channel photo multiplier); Decreased SNR
Time6 seconds for 96 wells independent of the number of dyes to be measured
Temperature Uniformity±0.1°C
WeightApprox. 30 kg
Lid TypeHeated Lid (30°C to 110°C)
Cooling RateUp to 6°C / sec (max.)
ApplicationsQuantitative real-time PCR; Multiplexing of up to 6 samples
Wells96 or 384 wells
Sensitivity1 nM FAM in minimal 30 µl sample volume
Electrical Requirements115V and 230V models available
Heating Rate8 °C/sec
Channels6

Features

Temperature gradient, Silver block, Connectivity (PC, Tablet)

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Local Office

Analytik Jena

3 Highwood Drive

Suite 103E

Tewksbury, MA 01876

United States

Phone: 1.800.452.6788

Email: info@us.analytik-jena.com

Company Profile

Website: https://www.analytik-jena.us/

International Office

Analytik Jena

Konrad-Zuse-Strasse 1

07745 Jena

Germany

Phone: +49 3641 77 7444

Company Profile

Website: https://www.analytik-jena.com/

(1) ETI signaling nodes are involved in resistance of Hawaii 7996 to Ralstonia solanacearum-induced bacterial wilt disease in tomatoPlant Signaling & BehaviorDecember 31, 2023Ai Xu, Lan Wei, Jingjing Ke, Chengfeng Peng, Pengyue Li, Changqiu Fan, Xiao Yu, Bo Ligreen Supermix (Monad, Cat. MQ00401S) with the gene-specific primers (Table S1) on the Analytik-jena qTOWER 3 System following standard protocols. The expression of each gene was normalized to the expression

(2) Unlocking the secrets of peptide transport in wine yeast: insights into oligopeptide transporter functions and nitrogen source preferencesApplied and Environmental MicrobiologyNovember 29, 2023Hidde Yaël Berg, Georg Arju, Carmen Becerra-Rodríguez, Virginie Galeote, Ildar NisamedtinovFIREPol EvaGreen qPCR Mix Plus (Solis BioDyne, Tartu, Estonia) was used. RT-PCR was conducted on a qTOWER G3 (Analytik Jena AG, Jena, Germany) system. A list of the primers used can be found in Table 3. Gene expression

(3) Vitexin Regulates Heat Shock Protein Expression by Modulating ROS Levels Thereby Protecting against Heat-Stress-Induced ApoptosisMoleculesNovember 17, 2023Tong Wu, Yanan Sheng, Yu Tian, Changyuan Wangquantitative polymerase chain reaction (RT-qPCR) using a TB Green Premix Ex Taq kit (Takara, Japan) and qTOWER instrument (Analytik Jena AG, Jena, Germany). mRNA expression levels were calculated using the 2−ΔΔCt

(4) Transcriptional deciphering of the metabolic pathways associated with the bioactive ingredients of wolfberry species with different quality characteristicsBMC GenomicsNovember 2, 2023Xuexia Liu, Rui Zheng, Yasmina Radani, Han Gao, Sijun Yue, Wenqiang Fan, Jianning Tang, Jing Shi, Jinzhong ZhuCo., Ltd, Wuhan, China), 0.4 μL of each primer and ddH2O. qPCR amplification was carried out on the qTOWER Real-time PCR Instrument (Analytik Jena AG, Germany). The following thermal cycling conditions were

(5) Maternal consumption of l-malic acid enriched diets improves antioxidant capacity and glucose metabolism in offspring by regulating the gut microbiotaRedox BiologyNovember 1, 2023Pengguang Zhang, Guoyuan Jiang, Yubo Wang, Enfa Yan, Linjuan He, Jianxin Guo, Jingdong Yin, Xin ZhangChina) according to the manufacturer's instructions. SybrGreen-based quantitative PCR was conducted in a qTOWER 2.2 thermocycler (Analytik Jena, Jena, Germany). GAPDH was used as the reference gene. The primers used

(6) Modelling TDP-43 proteinopathy in Drosophila uncovers shared and neuron-specific targets across ALS and FTD relevant circuitsActa Neuropathologica CommunicationsOctober 20, 2023R. Keating Godfrey, Eric Alsop, Reed T. Bjork, Brijesh S. Chauhan, Hillary C. Ruvalcaba, Jerry Antone, Lauren M. Gittings, Allison F. Michael, Christi Williams, Grace Hala’ufia, et al.Scientific Dm01798597_m1) and Gpdh1 (Thermofisher Scientific Dm01841185_m1). qPCR was conducted on the qTOWER (Analytik Jena 844-00504-4) qPCR machine. Delta Ct was calculated as the difference in Ct values (dlp—gpdh1).

(7) Uridine Diphosphate Glycosyltransferases (UGTs) Involved in the Carotenoid-Based Body Color Difference between Tetranychus cinnabarinus (Red) and Tetranychus urticae (Green)InsectsOctober 18, 2023Zhifeng Xu, Ting Lin, Tongyang Wang, Yuan Hu, Guangmao Shen, Kaiyang Feng, Ping Zhang, Lin Hethe internal reference genes (Table S1) [22]. qPCR was performed in 20 µL reaction mixtures using a qTOWER 2.0 (Analytik Jena, Jena, Germany) with the following cycling conditions: 95 °C for 2 min, then 40 cycles

(8) DNA extraction from recently fertilised Atlantic salmon embryos for use in microsatellite validation of triploidyPLOS ONEOctober 4, 2023Callum Howard, John B. Taggart, Caroline R. Bradley, Alejandro P. Gutierrez, John F. Taylor, Paulo A. Prodöhl, Herve Migaud, Michaël Bekaertfor the quality of bands and the presence of smear or primer dimer. The qPCR reactions were run on a QTower 3 (Analytik Jena, Germany) in accordance with the manufacturer’s instructions: Mix 1 μL of sample DNA

(9) Identification and evaluation of suitable reference genes for RT-qPCR analyses in Trichoderma atroviride under varying light conditionsFungal Biology and BiotechnologyOctober 3, 2023Daniel Flatschacher, Alexander Eschlböck, Susanne Zeilinger\usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\text {qTOWER}^3$$\end{document}qTOWER3 G cycler (Analytik Jena AG, Jena, Germany). All reactions except the ones

(10) Identification of a novel 5-methylcytosine-related signature for prognostic prediction of kidney renal papillary cell carcinoma and a Putative target for drug repurposingTranslational OncologyOctober 1, 2023Zhen Zhang, Chunhua Cao, Chun-Li Zhou, Xilong Li, Changhong Miao, Li Shen, Rajeev K. Singla, Xihua Lutranscriptase (Nanjing Vazyme Biotech Co., Ltd.). Quantitative real-time PCR (qRT-PCR) was performed using a qTOWER (Chen et al., 2020) G system (Analytik Jena, Germany) with SYBR Green to measure the mRNA expression

(11) Integrated meta-omics approaches reveal Saccharopolyspora as the core functional genus in huangjiu fermentationsnpj Biofilms and MicrobiomesSeptember 19, 2023Shuangping Liu, Zhi-Feng Zhang, Jieqi Mao, Zhilei Zhou, Jing Zhang, Caihong Shen, Songtao Wang, Maria L. Marco, Jian Maobacteria and fungi in wheat qu and HJFM according to the previous methods43. qPCR was performed using an qTOWER 3.0 system (Analytik Jena, Jena, Germany). DNA libraries were constructed using 1 μg genomic DNA and

(12) Analysis of the response mechanisms of Pinellia ternata to terahertz wave stresses using transcriptome and metabolic dataFrontiers in Plant ScienceSeptember 12, 2023Dongdong Wang, Surendra Sarsaiya, Xu Qian, Leilei Jin, Fuxing Shu, Chuanyou Zhang, Jishuang Chenas a reference to normalize the relative expression levels (Choi, 2020). qRT-PCR was performed using qTOWER real-time thermal cyclers from Analytik Jena in Germany. The relative expression rate was calculated

(13) Influence of High-Temperature and Intense Light on the Enzymatic Antioxidant System in Ginger (Zingiber officinale Roscoe) PlantletsMetabolitesSeptember 4, 2023Min Gong, Dongzhu Jiang, Ran Liu, Shuming Tian, Haitao Xing, Zhiduan Chen, Rujie Shi, Hong-Lei Lidefault [33]. 2.5. Expression Analysis of Enzyme Coding Genes by qRT-PCR The study utilized qRT-PCR (qTOWER 2.2, Analytik Jena AG, Germany) to assess the expression of enzyme genes under abiotic-stress conditions.

(14) Deficiency for scavenger receptors Stabilin‐1 and Stabilin‐2 leads to age‐dependent renal and hepatic depositions of fasciclin domain proteins TGFBI and Periostin in miceAging CellSeptember 1, 2023Thomas Leibing, Anna Riedel, Yannick Xi, Monica Adrian, Jessica Krzistetzko, Christof Kirkamm, Christof Dormann, Kai Schledzewski, Sergij Goerdt, Cyrill GéraudQuantitative PCR (qPCR) was performed using innuMIX qPCR SyGreen Sensitive (845‐AS‐1310200, Analytik Jena) on a qTOWER 3 G touch thermal cycler (Analytik Jena). Primers for RT‐qPCR were designed using NCBIs PrimerBLAST

(15) An insight into the temporal dynamics in the gut microbiome, metabolite signaling, immune response, and barrier function in suckling and weaned piglets under production conditionsFrontiers in Veterinary ScienceAugust 31, 2023Frederike Lerch, Fitra Yosi, Julia C. Vötterl, Simone Koger, Juliane Ehmig, Suchitra Sharma, Doris Verhovsek, Barbara U. Metzler-ZebeliWaltham, MA, United States). Absolute quantification of total microbes in fecal samples was performed on a qTOWER real-time PCR system (Analytik Jena GmbH, Jena, Germany) using previously published primer sets (Supplementary

(16) The differing effects of a dual acting regulator on SIRT1Frontiers in Molecular BiosciencesAugust 30, 2023Yujin Hur, Johnson Huynh, Emily Leong, Reena Dosanjh, Annemarie F. Charvat, My H. Vu, Zain Alam, Yue Tong Lee, Christiane C. Cabreros, Emma C. Carroll, et al.fluorimetry Differential Scanning Fluorimetry experiments were performed in a 384-well plate using a qTOWER real-time PCR thermal cycler (Analytik Jena). Fluorescence intensity was measured in the TAMRA channel

(17) Glutathione Transferases Are Involved in the Genotype-Specific Salt-Stress Response of Tomato PlantsAntioxidants (Basel)August 28, 2023Edit Horváth, Kitti Kulman, Bernát Tompa, Ádám Barnabás Hajnal, Alina Pelsőczi, Krisztina Bela, Ágnes Gallé, Jolán Csiszárquantitative real-time PCR (HT-qPCR, Avidin Ltd., Szeged, Hungary) and quantitative real-time PCR (qPCR, qTOWER Real-Time qPCR System, Analytik Jena, Jena, Germany) were applied to detect the expression of S. lycopersicum

(18) Icariin activates far upstream element binding protein 1 to regulate hypoxia-inducible factor-1α and hypoxia-inducible factor-2α signaling and benefits chondrocytesPeerJAugust 22, 2023Pengzhen Wang, Pingping Zhu, Shaoheng Zhang, Wei Yuan, Zhihe LiuKusatsu, Shiga, Japan). qPCR was performed with SYBR Premix ExTaq (Takara Bio, Kusatsu, Shiga, Japan) in a qTOWER 3G Real-time PCR system (Analytik Jena, Jena, Germany). Primer sequences used were as follows: FUBP1

(19) Transcriptome Analyses Reveal the Aroma Terpeniods Biosynthesis Pathways of Primula forbesii Franch. and the Functional Characterization of the PfDXS2 GeneInt J Mol SciAugust 12, 2023Yin Jia, Xiancai Yin, Hongchen Yang, Yuanfen Xiang, Keying Ding, Yuanzhi Pan, Beibei Jiang, Xue Yongwere designed using Primer Premier 5 software (Table S10). The qRT–PCR assay used an Analytik Jena qTOWER 2.2 fluorescence meter (CFXCONNECT, Bio-Rad, California, USA). The reaction program involved: denaturation

(20) Nutrients and soil structure influence furovirus infection of wheatFront Plant SciAugust 4, 2023Kevin Gauthier, Dejana Pankovic, Miroslav Nikolic, Mirko Hobert, Christoph U. Germeier, Frank Ordon, Dragan Perovic, Annette Niehlsubspecies after PCR or for detection of SBWMV or SBCMV after cDNA synthesis was performed using a qTower 2.2 (Analytik Jena) with 1 μL template, 500 nmol of each primer (1-BWF-6541T and 1-BWR-6683 for SBWMV,

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7/29/2015Real Time PCR with High Efficiency Muriel MalaiseWe are interested in minimum residual disease also called MRD. We used the qTOWER for our studies on MRD and we gained 2 points for Ct values.