Rad Source
RS-2000 Biological System
020712
I am conducting research on Parkinson’s disease, looking specifically at how effector T cells are able to modulate and exacerbate the neurodegeneration seen in the substantia nigra of Parkinson’s patients. The Rad Source RS-2000 Biological System is used in the irradiation of a single cell suspension of splenocytes.
Cell culture
Splenocytes
A single cell suspension of cells from the spleens of mice is made and red blood cell lysis is performed. Next, cells are placed on a level (1-5) of the irradiator and irradiated to 3600rad.
A longer radiation time on a level further from the source is better for cell survival than being close to the souce for a short period of time.
The cells are completely irradiated and complete cessation of cell proliferation is seen.
None
Cells are completely irradiated
Cell yield can be lowered if you use a level on the irradiator that is further from the source.
The Rad Source irradiator is a great machine for the irradiation of animal cells.