Cell Signaling Technology
EPAC1 (5D3) Mouse mAb
4155s
Info: View Product View Product Specs
Browse Similar Products: EPAC1 Antibodies
Western blot of extracts of whole mouse brain was done using EPAC1 antibody. We wanted to see whether the protein was expressed in NHBE cells but I was only able to do a control which is the band in the picture.
Western Blot
Mouse brain
1:1000 5% Milk in TBS1X-0.1% Tween20 overnight 4 degrees
5% Milk in TBS1X-0.1% Tween20 for 1 h RT
Goat anti mouse HRP 1:5000 in 5% Milk in TBS1X-0.1% Tween20 for 1 h RT
NA
Chemiluminescence
EPAC1 Western blot analysis from mouse brain lysate 2.5 ug at around ~100 KD
None
Maybe can dilute the antibody even further
Great product, maybe can dilute the antibody even further