Corning Life Sciences
HBSS, 1X
21-021-cv
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HBSS was used to extract spleens from mice and create a single cell suspension of splenocytes in HBSS media.
Cell culture
Mouse spleen
Spleens from mice are removed and placed into HBSS containing 1% FBS. Spleens are then crushed through mesh, and single cell suspension is created using a 5ml syringe and 18 gauge needle. The suspension is then centrifuged, red blood cells are lysed, cetrifuged again, and then brought up in HBSS for counting.
Addition of 1%FBS helps to create a more stable environment for the cells when they come out of the mouse.
The HBSS works as a great medium when harvesting mouse tissue or organs. It gives the user the ability to get the cells into culture in a timely manner without having a great loss of cells.
None
Cell counts are great every time.
If you dont add 1% FBS the cells tend to die faster in minimal HBSS.
Great to use when harvesting tissues, highly recommended!
Product Name: HBSS (Hank's Balanced Salt Solution), 1x with calcium and magnesiumSupplier Page