Great antibody and good specificity

I used this antibody to measure mesothelin expression on different cancer cell lines. I must say that the staining is pretty good and there is almost no background staining. I performed tests with multiple cell lines and a positive control (K562) that had transgenic expression of mesothelin. Upon analysis, I could clearly see the differences between the positive and negative cells (see flow image attached). The staining had to be standardized according to cell types. Cancer cells get a lot of background staining, but by using isotype control as well as gating out doublets in FSC-W and SSC-W plots, I was able to see very specific expression. Highly recommended for flow cytometry based assays. The company advertises for immunohistochemistry, but it works for flow as well.