Confocal Laser Scanning Microscopes

Confocal laser scanning microscopy (CLSM or LSCM) obtains high-resolution optical images by acquiring images point-by-point and reconstructing them with a computer, allowing three-dimensional reconstructions of topologically complex objects. These microscopes, found in biomedical clinical and research laboratories, are used in sensitivity demanding applications. There are two main subcategories- single point, often used in tissue slice imaging, and array scanning- often used for live cell imaging. When choosing a CLSM system, consider intended applications such as live-cell imaging or fixed-tissue sections, FRET, multiphoton microscopy or super-resolution imaging. Expanding into different types of imaging requires upgradability. Also important considerations in system selection are ease of use, light source, availability of maintenance contracts, and access to technical support.