Western blotting is a tried and true technique in the life science lab for separating proteins based on their molecular weight. Chemiluminescence is the most popular Western blot detection method because of its high level of sensitivity, and imaging systems geared toward use with the technique are available to further optimize speed, sensitivity, signal stability, and ease of use.
Automated image acquisition systems that combine techniques such as chemiluminescence, fluorescence, and bioluminescence offer enhanced flexibility. So, where to start in your search for the perfect system? The quality of the camera and the software, along with the level of automation, are the differentiating components. More tips:
- Check out the maximum magnification on the camera, and for proper comparison of zoom lenses, be sure all numbers refer to optical zoom
- Choose your optics: Lighting options include built-in UV transilluminators and RGB and white light sources
- Image acquisition and image analysis are two separate things, so double-check what’s included with your software.
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- Catalog Number: 81-12110-00
- Lighting: 365/302 nm pull out UV transilluminator / EPI white lights / EPI blue LEDs
- Resolution: 8MP
- Camera: 8MP camera with 16 bit A/D, regulated cooling to 50 C below ambient
- Dynamic Range: Wide dynamic range
- Applications: UV Imaging, White Light Imaging, Blue Light Imaging and Chemi Imaging
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Clinx Science Instruments
- Catalog Number: 10-500-40
- Lighting: EPI-White / (Optional EPI-Blue ,EPI-Green, EPI-Red LED)
- Camera: 4.2 Megapixel CCD Camera, cooled to -60° C below ambient
- Dynamic Range: 65,536 Grayscale
- Applications: Chemiluminescent Western Blot / Fluorescent Western Blot
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Clinx Science Instruments
- Catalog Number: 10-500-60
- Lighting: EPI-White / (Optional EPI-Blue ,EPI-Green, EPI-Red LED)
- Camera: 8.0 Megapixel CCD Camera, cooled to -60° C below ambient
- Dynamic Range: 65,536 Grayscale
- Applications: Chemiluminescent Western Blot / Fluorescent Western Blot
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- Catalog Number: 81-12100-00
- Lighting: UV Imaging, White Light Imaging, Blue Light Imaging and Chemi Imaging
- Resolution: High-resolution
- Camera: 6 megapixel, 16-bit camera, cooled 30ºC below ambient
- Dynamic Range: Wide dynamic range
- Applications: UV Imaging, White Light Imaging, Blue Light Imaging and Chemi Imaging
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- Catalog Number: 170-8265
- Lighting: Trans-UV Light / Epi White Light
- Camera: Supercooled CCD
- Dynamic Range: >4.0 Orders of Magnitude
- Applications: Chemiluminescence / Fluorescence / Colorimetry/ Densitometry / Gel Documentation
- Catalog Number:
- Lighting: epi-illumination
- Camera: CCD camera
- Dynamic Range: 4.0 to 5.0 OD
DNR Bio-Imaging Systems Ltd.
- Catalog Number: 70-25-00
- Lighting: Epi White Light
- Resolution: 2.0 Megapixels
4.2 Megapixels
11.0 Megapixels - Camera: CCD Camera
- Dynamic Range: >4.6 Orders of Magnitude
- Applications: ECL™, ECL-Plus™, Lumi Light Plus™, Super Signal™, CDP Star®, Luciferase and More
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GE Healthcare, Life Sciences
- Catalog Number: 63-0055-74
- Resolution: Autoradiography: 2 lp/mm
Blue-excited Fluorescence: 10 lp/mm
Green-excited Fluorescence: 10 lp/mm
Red-excited Fluorescence: 10 lp/mm - Dynamic Range: 5 OD
- Applications: Isotopic Imaging / Chemiluminescence / Blot Detection / Gel Stains / Solution Stains / Covalent Labels / Natural Fluorescence
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- Catalog Number: 3600
- Resolution: 196 µm x 196 µm
- Applications: The C-DiGit Blot Scanner was designed explicitly for chemiluminescent Western blotting.
- Catalog Number: 2800-01
- Lighting: 700 Channel Laser Source: Solid-state Laser Diode at 685 nm
800 Channel Laser Source: Solid-state Laser Diode at 785 nm - Dynamic Range: 22 bit (>6 logs)
- Catalog Number: 92-14860-00
- Lighting: UV Transilluminator / Epi White Light / Trans White Light
- Resolution: 8.3 Megapixels
- Camera: 8.3 Megapixels cooled CCD camera, 16-bit
- Dynamic Range: Up to 65,536 Grayscale
- Applications: Chemiluminescent / UV Fluorescent / Colorimetric / Colony Plates
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- Catalog Number:
- Lighting: 365/302 nm dual-wavelength,dual-intensity UV transilluminator, epi white lights
- Resolution: 1.4 Megapixels
- Camera: 1.4 Megapixels cooled CCD camera, 16-bit
- Applications: Chemiluminescent / Colorimetric / UV Fluorescent / Colony Plates
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- Catalog Number: 92-15407-00
- Lighting: Epi White Light
- Applications: Chemiluminescent / Colorimetric / UV Fluorescent / Colony Plates
- Catalog Number: 92-15409-00
- Lighting: Epi White Light
- Applications: Chemiluminescent / Colorimetric / UV Fluorescent / Colony Plates
- Catalog Number: 92-15408-00
- Lighting: Epi White Light
- Applications: Chemiluminescent / Colorimetric / UV Fluorescent / Colony Plates
- Catalog Number: 92-15410-00
- Lighting: Epi White Light
- Applications: Chemiluminescent / Colorimetric / UV Fluorescent / Colony Plates
- Catalog Number: 92-13603-00
- Lighting: 365/302 nm dual-wavelength, dual-intensity UV transilluminator, epi white lights and white light table.
- Resolution: 4.19 Megapixels
- Camera: 4.19 Megapixel cooled CCD camera, 16-bit
- Applications: Chemiluminescent / Colorimetric / UV Fluorescent / Colony Plates
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- Catalog Number: 92-15312-00
- Resolution: 8.3 Megapixels
- Camera: 8.3 Megapixels cooled CCD camera, 16-bit
- Dynamic Range: Up to 65,536 Grayscale
- Applications: Multiplex Westerns / Chemiluminescent Westerns / UV Fluorescent DNA and Protein Gels / Colorimetric Protein Gels / Colony Plates
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- Catalog Number: 92-14095-00
- Resolution: 4.19 Megapixels
- Camera: 4.19 Megapixel cooled CCD, 16-bit
- Applications: Multiplex Fluorescent Westerns / Chemiluminescent / Colorimetric / UV Fluorescent
- Catalog Number: 104250GR
- Resolution: 15.1 Megapixel
- Camera: Canon 40D digital SLR camera
- Applications: 96 well plate based chemiluminescent imaging/ chemiluminescent Western blot imaging
- Catalog Number:
- Lighting: White Light
- Resolution: 1.4m pixels up to 8.3m pixels
- Camera: Cooled CCD Camera
- Dynamic Range: 4.8 OD
- Applications: Fluorescence and chemiluminescence
- Catalog Number: 05-GGNOME-HR
- Lighting: Internal white light source
- Resolution: 5.0 Megapixels
- Camera: Cooled CCD Camera
- Dynamic Range: 4.8 Orders of Magnitude
- Applications: Chemiluminescence / Western Blots
- Catalog Number:
- Lighting: Lighting options include: UltraSlim Blue LED transilluminator, Lower White Light pad, Upper White LED lights, Red LED lights, Blue LED lights, Green LED lights, and IR LED lights.
- Camera: 4, 6 or 8 million pixel camera and suitable lens
- Applications: Wide range of applications: fluorescent and visible gels, chemiluminescent blots, fluorescent multiplex blots, fluorescent IR blots, and 2D gels
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- Catalog Number:
- Lighting: Epi White LED lights, Epi LEDs for multiplex or non-multiplex fluorescent blots, UltraSlim Blue LED transilluminator, Lower White Light pad
- Resolution: High resolution
- Camera: 4 million pixel camera
- Dynamic Range: High performance range
- Applications: Wide range of applications: fluorescent and visible gels, chemiluminescent blots, fluorescent multiplex blots, fluorescent IR blots, and 2D gels
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- Catalog Number:
- Lighting: Epi White LED lights, Epi LEDs for multiplex or non-multiplex fluorescent blots, UltraSlim Blue LED transilluminator, Lower White Light pad
- Resolution: High resolution
- Camera: 6 million pixel camera
- Dynamic Range: High performance range
- Applications: Wide range of applications: fluorescent and visible gels, chemiluminescent blots, fluorescent multiplex blots, fluorescent IR blots, and 2D gels
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- Catalog Number:
- Lighting: Epi White LED lights, Epi LEDs for multiplex or non-multiplex fluorescent blots, UltraSlim Blue LED transilluminator, Lower White Light pad
- Resolution: High resolution
- Camera: 8 million pixel camera
- Dynamic Range: High performance range
- Applications: Wide range of applications: fluorescent and visible gels, chemiluminescent blots, fluorescent multiplex blots, fluorescent IR blots, and 2D gels
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- Catalog Number: BioSpectrum 510
- Lighting: Epi white, UV illumination (365nm) and blue (480nm), choice of UV transilluminator
- Resolution: 4.1 megapixels w/fixed lens
- Camera: Cooled CCD Camera (BioChemi 510, OptiChemi 610 or MegaCam 810)
- Applications: Chemiluminescent, Fluorescent, Colorimetric
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- Catalog Number: BioSpectrum 610
- Lighting: Epi white, UV illumination (365nm) and blue (480nm), choice of UV transilluminator
- Resolution: 3.2 megapixels
- Camera: Cooled CCD Camera (BioChemi 510, OptiChemi 610 or MegaCam 810)
- Applications: Chemiluminescent, Fluorescent, Colorimetric
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- Catalog Number: BioSpectrum
- Lighting: Epi: white, UV illumination (365 nm) and 480 nm; UV transilluminator: select from single of multiple UV wavelengths and filter sizes from 20x20cm, 21x26cm or 25x26cm)
- Resolution: 3296 x 2472, 8.1 megapixels
- Camera: MegaCam 810 Monochrome CCD Cooled
- Applications: Chemiluminescence/ Western Blot/ Bioluminescence/ Plants/ Fluorescence/ Colorimetric/ 2D Gels/ GFP / Gels
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- Catalog Number: ChemiDoc-It 510/ ChemiDoc-It 610/ ChemiDoc-It 810
- Lighting: Epi White Illumination
- Resolution: BioChemi 510: 4.1 Megapixels with fixed lens
OptiChemi 610: 3.2 up to 9.6 Megapixels
MegaCam 810: 8.1 Megapixels - Camera: Cooled CCD Camera (BioChemi 510, OptiChemi 610 or MegaCam 810)
- Applications: Chemiluminescence / Western, Northern and Southern Blots
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