Excellent Alternative to Traditional Western Blotting

Miami
MI
Student

Overall

Performance

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Li-Cor

Product Name:

Odyssey CLx

Catalog Number:

9140-01

This system is used in conjunction with IR antibodies to perform western blots. This system allows to probe for multiple proteins at once due to the different color secondaries. Machine takes good pictures and comes with analysis software.

Experimental Design and Results Summary

Application

Western Blot

Starting Material

Probed western blot membrane

Protocol Overview

Load membrane with IR antibodies onto the CLx. Select western blot parameters e.g. quality and size of the scan area. Take the picture and then remove the membrane.

Tips

Minimize scan area to minimize image taking time

Results Summary

Western blots on this machine are quite easy and look pretty good. It also has the ability to probe multiple proteins at once, which is a time saver. The only drawback to this machine is the time it takes to produce an image. It can take 30 min. not even at the highest quality setting. Other than that it is a good alternative to traditional HRP western blots. Note: the secondary antibodies used will keep their signal for at least weeks unlike HRP.

Features Summary

Probe for multiple antibodies at once

Additional Notes

None

Image Gallery

Summary

The Good

Works well

The Bad

Takes a long time to take 1 image

The Bottom Line

This is a good machine for western blots.

Join the discussion