Expand High FidelityPLUS PCR System
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| Explore New Horizons in Amplification Yield |
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| Combine High Yield, Accuracy, and the Prevention
of Carry-Over Contamination |
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| Roche Applied Science, which pioneered the blending
of thermostable DNA polymerases with the Expand High Fidelity and Expand
Long Template PCR Systems, now introduces the Expand High FidelityPLUS PCR
System – the first product of a family of next-generation PCR Blends. |
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| This new blend combines Taq DNA Polymerase with
a novel proofreading protein isolated and characterized by Roche Applied
Science. This protein mediates proofreading activity, but has no polymerase
activity itself. |
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The synergy between the mechanism of the proofreading
protein and the processivity of Taq DNA Polymerase is the key to Expand
High FidelityPLUS Enzyme Blend’s unique ability to
- Deliver higher yields
- Improve accuracy for high-fidelity applications, and
- Incorporate dUTP to prevent carry-over contamination
for amplification reactions up to 5 kb.
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Improve the yield and accuracy of your current
amplification reaction
- Obtain higher yields than with any other polymerase or
mixture (Figure 1).
- Benefit from six-fold higher fidelity than with Taq DNA Polymerase
alone.
- Achieve greater sensitivity and specificity than with blends of Taq
and proofreading polymerases (Figures 1, 2).
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| Figure 1: Comparison of yield and accuracy
of various thermostable polymerases and blends. |
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| Combine the benefits of an enzyme
blend with the prevention of carry-over contamination for more reliable
results |
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| Carry-over contamination, in which the product
of a previous PCR erroneously serves as a template in a subsequent reaction,
is a problem in every laboratory. Carry-over contamination can be easily
prevented by using Taq polymerase to incorporate dUTP, then pretreating
all subsequent reactions with Uracil-DNA Glycosylase prior to cycling. |
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| However, since blends of Taq polymerase and
proofreading polymerases are incapable of incorporating dUTP, researchers
have traditionally been forced to choose between Taq polymerase’s ability
to incorporate dUTP and the polymerase blend’s greater yields and fidelity.
Taq polymerase only permitted the amplification of fragments up to ~3 kb,
making it impossible to prevent carry-over contamination for longer products. |
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| The Expand High FidelityPLUS Enzyme Blend has
changed all that. Use it to incorporate dUTP and amplify fragments up to
5 kb with greater yield and better fidelity (Figure 3). |
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Expand High FidelityPLUS PCR System*
Supplied with buffer that contains MgCl2, buffer without
MgCl2, and MgCl2 stock solution |
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| Cat. No. |
Pack Size |
| 3 300 242 |
125 units for 50 reactions |
| 3 300 226 |
500 units (2 x 250 units) for 200 reactions |
| 3 300 234 |
2,500 units (10 x 250 units) for 1,000 reactions |
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Don’t forget our PCR-Grade Nucleotides!
For maximum PCR sensitivity, insist on the highest purity
(>99% dNTP, <0.9% dNDP) nucleotides available. |
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| Product |
Cat. No. |
Pack Size |
| PCR Nucleotide Mix** |
1 581 295
1 814 362 |
100 reactions
1,000 reactions |
Set of Deoxynucleotides,
PCR Grade** |
1 969 064 |
4 x 25 µmol |
| PCR Nucleotide MixPLUS** |
1 888 412 |
2 x 100 µl, 200 reactions |
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