Brinkmann™/Eppendorf® SOP
Standard Operating Procedure for Pipettes
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Contents |
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1 Measuring station and accessories
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Test conditions in accordance with ASTM E-1154, DIN 12 650, ISO 8655,
and NCCLS (referred to as “The Standards”).
These test conditions are valid for all Eppendorf pipettes and dispensers
in the appropriate form. No special reference is made to the different tip
ejection mechanisms or the differences between manual and electronic pipettes.
Our expert staff offers cGMP/GLP service, in accordance with your SOP.
The following components are required for a measuring station for calibrating/adjusting
pipettes:
Balance tested by Board of Weights and Measures (NIST) (e.g. Sartorius,
Mettler, Ohaus or AnD)
Certified Barometer, Thermometer, and Hygrometer
Resolution/Accuracy:
The relationship between the error limits of the pipettes to be tested and
the graduation scale of the balance ensures that scattering within a measurement
series is recorded with sufficient accuracy to evaluate the accuracy and
precision in accordance with the DIN standard. Manufacturer specifications
sometimes deviate from the DIN standard and demand a high degree of weighing
accuracy (see “Technical specifications”).
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Nominal volume of the pipette
to be tested (µl) |
Error limits of the device
to be tested (µl) |
Required accuracy
of balance (g) |
| 1 – 50* |
0.1 – 0.8 |
0.00001 |
| > 50 – 1,000 |
1.0 – 10 |
0.0001 |
| > 1,000 |
> 10 |
0.001 |
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| * A balance with six decimal places or, where appropriate, a photometric
test should be used for volumes <= 1 µl. |
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Evaporation protection:
Moisture trap or other equipment which prevents evaporation.
Electrostatic charge:
For test volumes <= 50 µl, electrostatic discharge to all parts
which come in direct or indirect contact with the pipettes must be prevented.
The tubes provided by Eppendorf and the moisture trap with lid are made
of glass, which minimizes electrostatic charge.
Measuring station:
Calibration software and accessories for your balance (PICASO, catalog no.:
22 44 501-4) are recommended for electronic processing of measuring data.
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| Measuring station and accessories for test volumes |
Up to 50 µl |
50 µl and more |
| Balance setting |
int 1 (integration time)
asd 2 (standstill time) |
int 1
asd 2 |
| Weighing vessel, vessel adapter |
Vessel, diameter 15 mm (cat. no. 22 47 493-6, 3 pcs.) with vessel
adapter dia. 15 mm |
Vessel diameter 24 mm (cat. no. 22 47 492-8, 3 pcs.) with vessel
adapter dia. 24 mm |
| Evaporation protection |
Glass moisture trap |
Approx. 10 ml distilled water in a dish inside the balance |
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| For pipettes with a maximum volume of 5,000 µl and above,
the weighing plate with a vessel of an appropriate size, such as a 50 ml
volumetric flask, is used instead of the special Eppendorf balance adapter.
The moisture trap is not used in these cases. |
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2 Testing conditions and pipetting conditions
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Room temperature:
20 – 25°C, ± 0.5°C during measurement.
Temperature differences:
Factors which affect the temperature of the pipettes and measuring station
(e.g. direct sunlight) should be avoided.
Please ensure that the ambient temperature and the temperatures of the test
liquid and the pipettes used match the temperature of your tips. It is advisable
to temperature-stabilize all components for approximately 3 – 4 hours
prior to calibration.
Location of the balance:
The balance should be free of drafts and vibrations and should be placed
in a temperature-controlled environment.
Test liquid:
Degassed, bidistilled water which is at room temperature (20 – 25°C).
The water in the liquid supply or in the weighing vessel must be changed
every hour and must not be reused.
The humidity over the liquid surface of the weighing vessel should be maintained
at a value between 60% and 90% of the relative humidity.
Instruction manual:
In view of the many different types of volume measuring devices, it is particularly
important to refer to the instruction manual during testing. DIN 12 650,
Part 6 states that, in accordance with DIN 8418, the instruction manual
forms part of the delivery package of the device and the contents of the
instruction manual must be observed.
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3 Calibration of pipettes and bottletop dispensers/mode of operation
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3.1 Full Certification Procedure
(As performed at Eppendorf manufacturing facility and by special request
at Brinkmann Instrument, Inc.)
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Documentation supplied with certification includes:
- actual volume measurements for each measured volume
- standard calibration report
- pipette calibration sticker
Adjustable pipettes—three different volumes are tested:
- the nominal volume (10 measured values)
- approximately 50% of the nominal volume (10 measured values)
- the smallest adjustable volume, which should not be less than 10% of the
nominal volume (10 measured values)
Fixed-volume pipettes—the nominal volume is tested (10 measured
values).
Multichannel pipettes—the following volumes are tested for each channel:
- the nominal volume (3 measured values)
- approximately 50% of the nominal volume (3 measured values)
- the smallest adjustable volume, which should not be less than 10% of the
nominal volume (3 measured values)
Bottletop dispensers—the nominal volume is tested (10 measured values).
Repeater® Pipette—testing is carried out with the Eppendorf
Combitip® in Position 1 of the Repeater Pipette (48 measured values)
with a 12.5 ml Combitip.
Repeater Plus Pipette—testing is carried out with the Eppendorf Combitip
Plus Tip in Position 1 of the Repeater Plus Pipette (50 measured values)
with a 5 ml Combitip.
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3.2 Standard Calibration Procedure
(As performed as standard procedure at Brinkmann Instruments, Inc., Westbury,
NY facility)
Documentation supplied with calibration:
- standard calibration report
- pipette calibration sticker
Adjustable pipettes—three different volumes are tested:
- the nominal volume (5 measured values)
- approximately 50% of the nominal volume (5 measured values)
- the smallest adjustable volume, which should not be less than 10% of the
nominal volume (5 measured values)
Fixed-volume pipettes—the nominal volume is tested (5 measured values).
Multichannel pipettes—the following volumes are tested for each channel:
- the nominal volume (2 measured values)
- the smallest adjustable volume, which should not be less than 10% of the
nominal volume (2 measured values)
Bottletop dispensers—the nominal volume is tested (10 measured values).
Repeater® Pipette—testing is carried out with the Eppendorf
Combitip® in Position 1 of the Repeater Pipette (48 measured
values) with a 12.5 ml Combitip.
Repeater Plus Pipette—testing is carried out with the Eppendorf Combitip
Plus Tip in Position 1 of the Repeater Plus Pipette (50 measured values)
with a 5 ml Combitip.
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3.3 Brinkmann PACETM Calibration Procedure:
(As performed at customer account or through the PACE mail-in program)
Documentation supplied with Brinkmann PACE calibration includes:
- PACE calibration report
- pipette calibration sticker
Adjustable pipettes—two different volumes are tested:
- the nominal volume (4 measured values)
- the smallest adjustable volume, which should not be less than 10% of the
nominal volume(4 measured values)
Fixed-volume pipettes—the nominal volume is tested (4 measured values).
Multichannel pipettes—the following volumes are tested for each channel:
- the nominal volume (1 measured values)
- the smallest adjustable volume, which should not be less than 10% of the
nominal volume(1 measured values)
Repeater Pipette—fill Combitip, dispense 48 times (without measuring),
the steps must not skip, proceed to measure 4 weighings with a full Combitip
(Step# 48, 47, 46, & 45), 4 volumes with a half-full Combitip (Step#
25, 24, 23, & 22), 4 volumes at the low-volume Combitip (Step# 4, 3,
2, & 1).
Repeater Plus Pipette—fill Combitip, dispense 100 times (without measuring),
the steps must not skip, proceed to measure 4 weighings with a full Combitip
(Step# 100, 99, 98, & 97), 4 volumes with a half-full Combitip (Step#
51, 50, 49, & 48), 4 volumes at the low-volume Combitip (Step# 4, 3,
2, & 1).
Additional Services available:
- As Found Data available for an addition charge
- Proper Pipetting Technique Seminars: available to meet your cGMP/GLP training
requirements
Mode of operation: |
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Attach the appropriately-sized Eppendorf Pipette Tip
onto the nose cone of the pipette, or insert the appropriately-sized
Combitip into the Repeater pipette |
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Adjustable pipettes—set to the smallest volume
which is to be tested
Bottletop dispensers—set the nominal volume
Repeater pipettes—set the volume selection dial to Position 1
The first value of the Repeater Pipette is discarded (when the Combitip
is fully pulled). In the case of the Repeater Plus Pipette, the empty
stroke is executed (when the Combitip Plus Tip is fully pulled) |
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Tare the balance |
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Pre-wet the tip (not applicable for Combitips) |
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Aspirate and then dispense the set volume three times.
Execute blow-out |
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| Aspirate the volume which is to be tested from the liquid supply as
follows: |
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Hold the pipette vertically in the liquid supply |
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Immerse the tip approximately 2 – 3 mm into the
test liquid (see instruction manual) |
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Aspirate the test volume slowly and uniformly. Observe
the waiting period of 1 – 3 sec. (for the Series 2100 (Research),
500 – 5,000 µl: 5 sec.)
(The waiting time depends on the size of the pipette tip; see instruction
manual) |
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Remove the pipette tip from the test liquid slowly and
uniformly. Remove any remaining liquid by “wiping” the pipette
tip against the inside of the vessel |
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| Dispense the test volume into the weighing vessel as follows: |
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Place the filled tip at an angle of 30° against
the inside of the weighing vessel |
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Dispense the test volume slowly and uniformly up to
the first stop (measuring stroke) and wait for 1 – 3 sec. (This
applies to manual pipettes only) |
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Press the control button to the second stop (blow-out)
and dispense any liquid remaining in the tip (not applicable when
bottletop dispensers and the Repeater pipettes are used) |
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Hold down the control button and pull the tip up along
the inside of the weighing vessel |
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Document the value which appears in the display of the
balance immediately after the display has come to a standstill |
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Document all measured values from a measurement series
as described above. Evaluate the inaccuracy and the imprecision |
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With adjustable pipettes, proceed in the same way, using
the nominal volume and 50% of the nominal volume |
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With multichannel pipettes, the measured values of all
channels per test volume are summarized for evaluation of the inaccuracy
and imprecision |
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| Please note: When user testing, follow section 3.1 Full Certification
Procedure. |
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4 Individual evaluation
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| The following calculation is carried out from all measured values within
the evaluation limits: |
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| Mean value |
= x |
| Number of measured values |
= n |
| Standard deviation |
= s |
| Coefficient of variation |
= CV (imprecision) |
| Inaccuracy |
= d |
| Range |
= Difference between maximum and minimum value |
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For exact calculation, Z factor is used to correct changes in the test
liquid regarding temperature and air pressure.
The manufacturer specifications for the pipette can be found in the instruction
manual or in “Technical specifications” |
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5 Sterilizing and cleaning the pipette
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Sterilization
The instruction manual of the pipette contains information on cleaning with
solvents or autoclaving(121 °C, 20 min.).
Eppendorf pipettes should be autoclaved as follows:
The Model 4800 and the Model 4810, the Series 2000 (Reference),
fixed-volume and adjustable-volume, and the Positive Displacement
Pipette (Model 4830) are fully autoclavable.
Before autoclaving, unscrew the upper housing from the lower housing of
the pipette by rotating the central junction once. This allows vapor to
enter the device easily. Do not reassemble until the pipette cools down
to room temperature.
Only the lower parts of the Series 2100 (Research) and Multichannel
Pipettes are autoclavable.
Series 2100 (Research): The tip ejector is pulled off the pipette and
the lower part of the pipette is unscrewed. The lower part and the ejector
can now be autoclaved. Do not reassemble the parts until they have reached
room temperature.
Titermate: Turn the control button to the uppermost stop. Press your
thumb against the grip of the pipette and push the lower part forward through
the slit. Do not reassemble the parts until they have reached room temperature.
Brinkmann Bottletop Dispenser: must be completely disassembled before
autoclaving begins.
Brinkmann ChemSaver® Dispenser: Move the discharge
valve toggle into the dispensing position (->). Disengage the volume
setting button, push it into the middle position and leave it disengaged.
Place the dispenser onto a cloth and autoclave the device. Ensure that the
device does not come into contact with hot metal surfaces. Do not use the
dispenser until it has cooled down to room temperature.
The Series 2100 (Research), fixed-volume, the MaxipettorTM,
the Repeater Pipette, and the Repeater Plus Pipette are not
autoclavable.
Cleaning:
If not otherwise stated in the instruction manual, all parts of the pipette
can be cleaned with a soap solution or isopropanol. The parts must then
be rinsed with distilled water. Do not reassemble the parts until they have
dried completely.
After cleaning, manual pipettes should be lubricated lightly using the special
silicone grease included with the pipette. (To reorder: catalog no.: 22
34 851-5) |
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6 Checking for leaks
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For pipettes with volumes 100 µl and up:
To check for leaks, hold the pipette vertically for approximately 15 seconds
with a filled tip. Do not touch the pipette tip. Observe the meniscus of
the liquid in the tip. If there is a leak, a droplet is visible on the pipette
tip.
For all volume pipettes:
Special equipment can be used to test for leaks in the system, such as the
Brinkmann Instruments Universal Pipette Tester (catalog no:. 22 47 490-3). |
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7 Troubleshooting
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| (See instruction manual for further details) |
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| Error |
Cause |
Solution |
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| Droplets visible on the inside of the pipette tip |
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Uneven wetting of the plastic wall |
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Attach a new pipette tip |
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| Pipette drips; incorrect pipetting volume |
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Tip is loose |
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Incorrect pipette tip |
| Pipette leaks because: |
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the piston is contaminated |
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the piston is damaged |
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the seals are damaged |
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the nose cone is loose |
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Push tip on tightly |
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Use Eppendorf Pipette Tip |
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Clean the piston and lubricate lightly |
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Replace the piston and piston seals and lubricate
lightly |
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Replace all seals and lubricate the piston lightly |
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Tighten the nose cone slightly using the key provided;
replace the nose cone if necessary |
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Air bubble present in Combitip Plus; liquid cannot be
aspirated correctly;
dripping occurs |
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Combitip Plus Tip is leaking |
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Replace the Combitip Plus Tip |
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Temperature of Combitip Plus Tip has risen |
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Ensure that the temperature is uniform as liquid
expands when heated |
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| Control button jams or does not move smoothly |
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Piston is contaminated |
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Seal is contaminated |
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Piston is damaged |
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Solvent vapors have entered device |
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Clean the piston and lubricate lightly |
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Disassemble the pipette; clean all seals and replace
if necessary; lubricate the piston lightly |
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Replace the piston and piston seals and lubricate
lightly |
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Unscrew the pipette at the central junction and
air the pipette. Clean the piston and lubricate lightly |
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8 Adjustment
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The pipettes have been calibrated by the manufacturer with bidistilled,
degassed water in accordance with the Standards under the measuring
conditions described in Section 2.
If the precision of the pipetted volume is questionable, consider the following
points before readjusting the pipette:
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Has the pipette been checked for leaks? (see Sec. 7)
Exception: Positive Displacement Pipette (Model 4830). |
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Is the temperature of the liquid the same as:
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the temperature of the device? |
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the ambient temperature? |
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Is there a draft in the room? |
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Has the correct volume been set? |
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Have the density of the liquid and the air pressure
been taken into consideration? |
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Does the density of the test liquid deviate from that
of bidistilled, degassed water? |
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Is the mode of operation correct? (see Sections 3 and
4) |
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Have Eppendorf Pipette Tips been attached (pipette +
pipette tip -> pipetting system)? |
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Volume errors may occur during the pipetting of liquids which have a high
vapor pressure or a density which is significantly different to that of
water.
Do not adjust the device until all of these points have been examined. |
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8.1 Adjusting pipettes (adjustable-volume)
The adjustment of adjustable-volume pipettes Series 2100 (Research),
Model 4810, Series 2000 (Reference)
adjustable-volume, Positive Displacement Pipette (Model 4830), and Titermate
is a zero-point shift.
- In accordance with the Standards, Part 6; the temperature of the device,
the Eppendorf Pipette Tip, the test liquid, and the ambient conditions
must be the same (20 – 25 °C constant to ± 0.5 °C)
during testing.
- Set the device to the smallest possible volume, which should not be
less than 10% of the nominal volume.
- When using single-channel pipettes, attach an appropriate Eppendorf
Pipette Tip to the pipette. With multichannel pipettes, the tip can
be attached to any one channel.
- Pipette the volume ten times. The actual volume is calculated from
the mean value obtained from these weighings (use the Z factor to convert
mg into µl).
- To adjust the device, insert the tool provided into the appropriate
opening and set the actual volume (see Fig. 1 – 8 or consult the
instruction manual).
- Check the volume which has been set by carrying out a measurement
once again. If the nominal volume does not correspond with the result
of the measurement, repeat points 2 – 5.
- After adjustment, check the accuracy of the measured values of the
nominal volume and of 50% of the nominal volume.
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8.2 Adjusting pipettes (fixed-volume)
Fixed-volume pipettes are adjusted in the same way as adjustable-volume
pipettes (with fixed-volume pipettes,
10 measured values of the nominal volume are checked).
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Adjusting the Series 2000 (Reference),
fixed-volume: |
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To facilitate relocation of the initial setting, attach the
label provided (calibration aid) to the control button, as described
in the instruction manual.
Using side B of the universal tool provided, loosen the inner
screw until the control button can be rotated (see Fig. 1).
Adjust the control button to the volume which has been obtained
under point 4 (see instruction manual). One revolution of the
control button is equivalent to the following (figures based
on water):
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| Reference fix |
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Volume/rotation |
| 1, 2, 5, 10 µl |
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approx. 0.5 µl |
| 10, 20 µl |
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approx. 1 µl |
| 25, 50 µl |
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approx. 2.4 µl |
| 100 µl |
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approx. 5 µl |
| 200, 250 µl |
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approx. 12 µl |
| 500, 1,000 µl |
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approx. 46 µl |
| 1,500, 2,000, 2,500 µl |
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approx. 118 µl |
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| Adjusting the Series 2100
(Research), fixed-volume: |
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To align to the volume displayed, insert side
D of the wrench horizontally into the alignment opening (Fig.
2) in the pipette grip. (When doing so, hold the pipette vertically.)
Rotate the wrench into a vertical position.
To reduce the volume, turn the setting ring clockwise. To increase
the volume, turn the setting ring counterclockwise. One rotation
is equal to the following:
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| Reference fix |
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Volume/rotation |
| 5 µl |
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approx. 0.8 µl |
| 10µl |
|
approx. 0.8 µl |
| 20µ |
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approx. 0.8 µl |
| 25µl |
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approx. 4 µl |
| 50 µl |
|
approx. 4 µl |
| 100 µl |
|
approx. 4 µl |
| 200 µl |
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approx. 38 µl |
| 250 µl |
|
approx. 38 µl |
| 450 µl |
|
approx. 38 µl |
| 500 µl |
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approx. 38 µl |
| 1,000 µl |
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approx. 38 µl |
Remove the wrench and move the setting ring backwards
and forwards until the counter and the stroke system lock
together. |
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| Adjusting the Series 2000 (Reference),
adjustable-volume: |
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Turn the universal tool provided to set the digital
indicator of the pipette to its actual volume (the measurement
in point 4). The piston stroke should remain unchanged. See
Fig. 3.
Remove the tool. Set the Series 2000 (Reference) to its nominal
volume in the usual way (see instruction manual).
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| Adjusting the Series 2100 (Research
single and multichannel) adjustable-volume: |
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Insert side D of the key provided horizontally
into the upper half of the calibration opening at the side of
the pipette grip (see Fig. 4). Move the key into a vertical
position.
When the setting ring is rotated (either in the + or - direction),
the piston stroke of the pipette is altered (although the volume-setting
dial remains unchanged).
One rotation is equal to the following:
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| Research, adjustable-volume |
| 0.1-2.5 µl |
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approx. 0.1 µl |
| 0.5-10 µl |
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approx. 0.1 µl |
| 2-20 µl |
|
approx. 0.8 µl |
| 10-100 µl |
|
approx. 4 µl |
| 20-200 µl |
|
approx. 4 µl |
| 100-1,000 µl |
|
approx. 4 µl |
| 500-5,000 µl |
|
approx. 38 µl |
| Research, multichannel |
| 0.5-10 µl |
|
approx. 0.5 µl |
| 10-100 µl |
|
approx. 5 µl |
| 30-300 µl |
|
approx. 10 µl |
After removing the key, move the adjusting ring to and
fro until the digital indicator system and the stroke
system click together (see instruction manual). |
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| Adjusting the Model 4810: |
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Using a Phillips screwdriver (size 0), set the
digital indicator of the pipette to the actual volume (the measurement
in point 4). Make sure that the control button is fully pushed
in. See Fig. 5.
Remove the screwdriver. Set the Model 4810 to its nominal volume
in the usual way (see instruction manual).
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| Adjusting the Positive Displacement
Pipette (Model 4830): |
|
Turn the universal tool provided to set the digital
indicator of the pipette to its actual volume (the measurement
in point 4). The piston stroke should remain unchanged. See
Fig. 6.
Remove the tool. Set the Positive Displacement Pipette to its
nominal volume in the usual way (see instruction manual).
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| Adjusting the Titermate: |
|
Using a Phillips screwdriver (size 0), set the
digital indicator of the pipette to the actual volume (the measurement
in point 4). Make sure that the control button is fully pushed
in. See Fig. 7.
Remove the screwdriver. Set the Titermate to its nominal volume
in the usual way (see instruction manual). |
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| Adjusting the ChemSaver
Bottletop Dispenser: |
Adjust the ChemSaver Bottletop Dispenser by turning
the fine adjustment (see Fig. 8). One rotation is equivalent
to the smallest dispensing step.
Rotation of the button in the “+” direction: increase
in volume.
Rotation of the button in the “–” direction:
decrease in volume.
(Factory-set adjustment at 20 °C with degassed, bidistilled
water; see instruction manual.)
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| 8.3 Adjusting for liquids with a density different to that of bidistilled,
degassed water |
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It is possible to adjust the pipettes described on the previous pages
for one volume of a liquid with a density different to that of water so
that the volume displayed corresponds with the pipetted volume.
Caution: With adjustable pipettes, all other values are then adjusted
as well!
The adjustment process is similar to the procedure described in Sec. 8.1
and 8.2. The difference is the mean value of the weighings is converted
into microliters using the following formula:
|
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Mean value of weighings |
| Pipetting volume= |
|
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Density of liquid weighed |
|
The resulting value is the actual value to which the volume display of adjustable-volume
pipettes or the volume of fixed-volume pipettes is adjusted.
Caution: The value set for this particular liquid must be checked
gravimetrically. A pipette set in this way only provides a value corresponding
with the digital indicator display/volume ring for the liquid used and for
the volume set. If necessary, the setting must be corrected and checked
again.
After the pipette has been adjusted to another value, the device must be
clearly identifiable (e.g. a label should be applied). |
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9 Factor Z for distilled water
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Factor Z (µl / mg) in accordance with ISO DIS 8655/3 as a function
of the temperature and air pressure for distilled water
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10 Technical specifications of Eppendorf pipettes
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10.1 Fixed-volume pipettes
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10.2 Adjustable pipettes
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| Liquid: |
Bidistilled water |
| Reference temperature: |
20 – 25 °C, constant to ± 0.5 °C |
| Number of determinations: |
10, in accordance with the Standards
with Eppendorf Pipette Tips |
|
|
| Liquid: |
Bidistilled water |
| Reference temperature: |
20 – 25 °C, constant to ± 0.5 °C |
| Number of determinations: |
10, in accordance with the Standards
with Eppendorf Pipette Tips |
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| 10.3 Multichannel pipettes |
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 |
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| Liquid: |
Bidistilled water |
| Reference temperature: |
20 – 25 °C, constant to ± 0.5 °C |
| Number of determinations: |
10, in accordance with the Standards
with Eppendorf Pipette Tips |
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| 10.4 Repeater Plus Pipette |
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 |
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| 10.5 Bottletop dispensers |
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 |
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| Liquid: |
Bidistilled water |
| Reference temperature: |
20 – 25 °C, constant to ± 0.5 °C |
| Number of determinations: |
10, in accordance with the Standards
with Eppendorf Pipette Tips |
|
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| 10.6 Error limits in accordance with DIN 12 650 |
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In accordance with DIN 12 650, the error limits are always based
on the overall system of pipette and tip together. The error limits of pipettes
with a nominal volume between the nominal volumes given in the table must
correspond to the relative error limits for the next-highest nominal volume.
If the nominal volume of the pipette is exactly between two nominal volumes
given in Table 1, the relative error limit for the lower volume is applicable.
The error limits in accordance with the latest version of the DIN 12 650
standard and based on volume are contained in the following tables: |
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 |
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The error limit in accordance with the DIN standard is defined by F =
IAI + 2 s, whereby:
| F |
= |
Error limit |
| |A| |
= |
Systematic measurement deviation
(Deviation of the mean value of 10 measurements of the nominal volume
= Inaccuracy) |
| s |
= |
Random measurement deviation
(Repeated standard deviation of the 10 measurements = Imprecision) |
|
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Reference sources for pipetting standards
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Information concerning proper pipetting technique and gravimetric testing
procedure can be obtained by requesting copies of ISO (International Standard
Organization) or DIN (Deutsche Industrial Norm) Standards from the American
National Standard Institute (ANSI).
Request designation number ISO 8655 or DIN 12 650
ANSI
11 West 42nd Street
New York, NY 10036
Telephone: (212) 642-4900; Fax: (212) 398-0023
E-mail: info@ansi.org
Also, the ASTM Standard can be requested by contacting:
ASTM (American Society for Testing and Materials)
100 Barr Harbor Drive
West Conshohocken, PA 19428-2959
Telephone: (610) 832-9585; Fax: (610) 832-9555
E-mail: service@astm.org
Request designation number E 1154-89 “Standard Specification for Piston
or Plunger Operated Volumetric Apparatus.”
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