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Application Notes
Optimizing Electroporation Parameters
Optimizing Electroporation Parameters
Optimizing electroporation parameters
To ensure that maximum transfection rates are achieved, the electroporation parameters should be optimized for each new cell line. The guidelines listed below for determining the ideal electroporation parameters as simply and as quickly as possible are adapted from Eppendorf
®
's Multiporator
®
Brochure available upon request.
Field strength
Pulse length
Number of field pulses
Adjustment of the electroporation buffer
Influence of DNA/RNA
Influence of temperature
Influence of the cell density
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Read more Application Notes from Eppendorf North America
Eppendorf Contact Information
Eppendorf
In the United States:
Eppendorf North America, Inc.
102 Motor Parkway,
Hauppauge, NY 11788-5178
Outside the United States:
Eppendorf AG
Barkhausenweg 1
22339 Hamburg
Germany
Tel: ++ 49 40 53 801-0
Fax: ++ 49 40 53 801-556
Web Site: http://www.eppendorf.com
Customer Service: 800-645-3050
Fax Number: 516-334-7506
Web Site:
http://www.eppendorfna.com
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