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Synthesize mRNA Transcripts In Vitro With More Confidence
Ambion's mMESSAGE mMACHINE™ High Yield Capped RNA Transcription Kit uses patented technology to synthesize large mass amounts of capped RNA for microinjection, transfection and in vitro expression studies. We have recently made an improvement to the mMESSAGE mMACHINE Kit that significantly improves the protection of synthesized RNA from inadvertent ribonuclease contamination. The format of the kit has remained unchanged, but the antiquated placental ribonuclease inhibitor (RIP) has been replaced with SUPERase•In™, a novel broad-spectrum RNase Inhibitor. Why Change Inhibitors? The addition of SUPERase•In makes the mMESSAGE mMACHINE Kit even more robust than before. The ribonuclease inhibitor typically used in in vitro transcription reactions is RIP. RIP only inhibits RNase A and its carbohydrate variants, RNases B and C. SUPERase•In not only inhibits these RNases, but it also inhibits RNases 1 and T1, bacterial RNases that can be carried over from template DNA preparations. When you consider where RNase contamination might originate, it becomes clear why you need to inhibit different types of RNases. In addition, SUPERase•In is stable over a broader range of conditions than RIP. It protects synthesized RNA against a greater concentration of RNases, and over a wider pH range. The additional protection provided by SUPERase•In offers the researcher an extra level of assurance that RNases will not degrade one's RNA, and that full-length proteins will be synthesized during the downstream translation reaction. |