CellTiter-Glo Luminescent Cell Viability Assay G7572 from Promega

Product Specs
ItemCellTiter-Glo Luminescent Cell Viability Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG7572
Detection TargetCell viability
Assay Time10 minutes
Sample TypeLive Cells
Quantity100ml
Detectionluminescence
MethodLuminescent

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Augmented Concentration of Isopentyl-Deoxynyboquinone in Tumors Selectively Kills NAD(P)H Quinone Oxidoreductase 1-Positive Cancer Cells through Programmed Necrotic and Apoptotic MechanismsCancers (Basel)December 14, 2023Jiangwei Wang, Xiaolin Su, Lingxiang Jiang, Matthew W. Boudreau, Lindsay E. Chatkewitz, Jessica A. Kilgore, Kashif Rafiq Zahid, Noelle S. Williams, Yaomin Chen, Shaohui Liu, et al.points using respective standard chemical assays according to the manufacturer’s protocols (Promega G7572, Promega G8820, and Promega G9071) [21]. 2.8. Immunofluorescence Assay To assess the possibility of

(2) A novel microtubule inhibitor promotes tumor ferroptosis by attenuating SLC7A11/GPX4 signalingCell Death DiscovDecember 13, 2023Nannan Ning, Ziqi Shang, Zhiping Liu, Zhizhou Xia, Yang Li, Ruibao Ren, Hongmei Wang, Yi Zhangto the cells, and the cells were incubated in an incubator for 1 h. CellTiter-Glo Reagent (Promega, G7572) was added to each well, and the 96-well plate was incubated at room temperature for 10 min. Then ATP

(3) Tumor-selective effects of active RAS inhibition in pancreatic ductal adenocarcinomabioRxivDecember 4, 2023Urszula N. Wasko, Jingjing Jiang, Alvaro Curiel-Garcia, Yingyun Wang, Bianca Lee, Margo Orlen, Kristina Drizyte-Miller, Marie Menard, Julien Dilly, Stephen A. Sastra, et al.adenosine triphosphate (ATP) levels using the CellTiter-Glo® Luminescent Cell Viability Assay (Promega, G7572) according to the manufacturer’s instructions. Technical triplicates were run for each biological replicate

(4) GNF-7, a novel FLT3 inhibitor, overcomes drug resistance for the treatment of FLT3‑ITD acute myeloid leukemiaCancer Cell InternationalNovember 30, 2023Xinhua Xiao, Peihong Wang, Weina Zhang, Jiayi Wang, Mansi Cai, Hua Jiang, Yingli Wu, Huizhuang Shanvarious concentrations. After 48 h incubation, cells were lysed by CellTiter Glo reagent (Promega, #G7572) and the luminescence signals were detected through a multimode microplate reader (VICTOR Nivo). Cell

(5) PDE3A Is a Highly Expressed Therapy Target in Myxoid LiposarcomaCancers (Basel)November 7, 2023Kirsi Toivanen, Sami Kilpinen, Kalle Ojala, Nanna Merikoski, Sami Salmikangas, Mika Sampo, Tom Böhling, Harri Sihtoday. Cell viabilities were measured with CellTiter-Glo® Luminescent Cell Viability Assay (Cat. No. G7572, Promega, Madison, WI, USA), following the manufacturer’s instructions. Luminescence was recorded with

(6) Therapeutic Advantage of Targeting PRMT5 in Combination with Chemotherapies or EGFR/HER2 Inhibitors in Triple-Negative Breast CancersBreast Cancer: Targets and TherapyNovember 6, 2023Rayan Dakroub, Solène Huard, Yara Hajj-Younes, Samyuktha Suresh, Bassam Badran, Hussein Fayyad-Kazan, Thierry Dubois(M2128-1G, Sigma-Aldrich, St. Louis, MO, USA), WST-1 (11,644,807,001, Sigma-Aldrich), or CellTiterGlo (G7572, Promega, Madison, WI, USA) after four doubling times (3–7 days depending on the cell line) (Table S1). For

(7) The E301R protein of African swine fever virus functions as a sliding clamp involved in viral genome replicationmBioOctober 31, 2023Su Li, Hailiang Ge, Yanhua Li, Kehui Zhang, Shaoxiong Yu, Hongwei Cao, Yanjin Wang, Hao Deng, Jiaqi Li, Jingwen Dai, et al.ectopically expressed pE301R upon PCNA knockdown was analyzed using a CellTiter-Glo kit (catalog no. G7572, Promega) according to the manufacturer’s instructions. To determine the maximum non-cytotoxic concentration

(8) ATP synthase-associated coiled-coil-helix-coiled-coil-helix (CHCH) domain-containing proteins are critical for mitochondrial function in Toxoplasma gondiimBioOctober 31, 2023Madelaine M. Usey, Diego Huetdetermine the ATP concentration in each condition, 100 µL of CellTiter-Glo reagent (Promega, cat. no. G7572) was added to the wells while samples thawed at room temperature for 1 h. After thawing, 100 µL of each

(9) Prolonged hydrogen production by engineered green algae photovoltaic power stationsNature CommunicationsOctober 25, 2023Hyo Jin Gwon, Geonwoo Park, JaeHyoung Yun, WonHyoung Ryu, Hyun S. Ahndry weight (g). Cell viability analysis Cell viability was assessed using CellTiterGlo® (Promega, G7572). 500 μL cultures of C. reinhardtii - CNF/Pt from the hydrogen production sample were centrifuged (900

(10) Small molecule targeting of transcription-replication conflict for selective chemotherapyCell chemical biologyOctober 19, 2023Gu L, Li M, Li CM, Haratipour P, Lingeman R, Jossart J, Gutova M, Flores L, Hyde C, Kenjić N, et al.compounds: AOH1996, AOH1996-1LE, and AOH1160-1LE",This paper,N/A CellTiter-Glo assay,Promega,Cat# G7572 Kolliphor EL,Sigma,Cat# C5135 Poloxamer P124,Spectrum Chemical Mfg. Corp.,Cat# P1168 Crystal Violet,Sigma,Cat#

(11) Guide-specific loss of efficiency and off-target reduction with Cas9 variantsNucleic Acids ResearchOctober 13, 2023Liang Zhang, Wei He, Rongjie Fu, Shuyue Wang, Yiwen Chen, Han Xuto selection with Puro (2 μg/ml). Cell viability was tested using CellTiter-Glo® reagent (Promega, #G7572) 3 days after selection and the virus titer was determined based on the cell survival rate. Stable

(12) Polarity protein AF6 functions as a modulator of necroptosis by regulating ubiquitination of RIPK1 in liver diseasesCell Death & DiseaseOctober 12, 2023Wang Xinyu, Wen Qian, Wu Yanjun, Kong Jingwen, Xu Keying, Jiao Jiazheng, Zhang Haibing, Wang Kai, Xu Xiao, Zhan Lixingassay Cell survival was assessed using the Cell Titer-Glo Luminescent Cell Viability Assay kit (Promega Cat. G7572) and the luminescence was measured with an Empire microplate reader. Administration of recombinant

(13) Inducing Mitotic Catastrophe as a Therapeutic Approach to Improve Outcomes in Ewing SarcomaCancersOctober 10, 2023Turaga SM, Vishwakarma V, Hembruff SL, Gibbs BK, Sabu P, Puri RV, Pathak HB, Samuel G, Godwin AK.were incubated for 72 h before cell viability readings were measured. CellTiter-Glo reagent (Promega # G7572) mixed with Glo Lysis buffer (Promega # E2661) in a 1:2 ratio was used for analysis. An equal volume

(14) Multi-step processing of replication stress-derived nascent strand DNA gaps by MRE11 and EXO1 nucleasesNature communicationsOctober 7, 2023Hale A, Dhoonmoon A, Straka J, Nicolae CM, Moldovan GL.drug treatment, a luminescent ATP-based assay was performed using the CellTiterGlo reagent (Promega G7572) according to the manufacturer’s instructions. Following treatment with siRNA, 1500 cells were seeded

(15) MET exon 14 skipping is overexpressed in an allele-specific manner in lung adenocarcinoma primary samplesMicroPubl BiolSeptember 26, 2023Megan Durham, Swetha Vadde, Angela N Brooksassessed in order to normalize Day 8 to the initial plating concentration. First, Cell Titer Glo (Promega, G7572) was allowed to come to room temperature. Then, a 1:1 ratio of Cell Titer Glo was added to the cells

(16) Design principles for cyclin K molecular glue degradersNature Chemical BiologySeptember 7, 2023Zuzanna Kozicka, Dakota J. Suchyta, Vivian Focht, Georg Kempf, Georg Petzold, Marius Jentzsch, Charles Zou, Cristina Di Genua, Katherine A. Donovan, Seemon Coomar, et al.days of drug exposure, cell viability was assessed using the CellTiter-Glo luminescent assay (Promega, G7572) on an CLARIOstar Plus, MARS 3.4 (BMG LabTech). Cell viabilities were calculated relative to DMSO controls.

(17) N6-Methyladenosine-Modified ATP8B1-AS1 Exerts Oncogenic Roles in Hepatocellular Carcinoma via Epigenetically Activating MYCJournal of Hepatocellular CarcinomaSeptember 6, 2023Chuan Tan, Yanyan Huang, Zheng Huang, Yuanjia Ning, Lizheng Huang, Xianjian Wu, Yuan Lu, Huamei Wei, Jian Pucell viability assay was performed using the CellTiter-Glo Luminescent Cell Viability Assay (cat. no. G7572, Promega, Madison, WI, USA) after culture for 48h as previously reported.43 The luminescence was used

(18) Targeting glutamine metabolic reprogramming of SLC7A5 enhances the efficacy of anti-PD-1 in triple-negative breast cancerFrontiers in ImmunologySeptember 4, 2023Renhong Huang, Han Wang, Jin Hong, Jiayi Wu, Ou Huang, Jianrong He, Weiguo Chen, Yafen Li, Xiaosong Chen, Kunwei Shen, et al.Cell viability assay Cell viability was measured with a CellTiter-Glo® Luminescent Cell Viability Assay (Promega, Cat # G7572, Lot # 268755) in accordance with the manufacturer’s instructions. Briefly, cells were seeded onto 96-well

(19) Inhibiting NINJ1-dependent plasma membrane rupture protects against inflammasome-induced blood coagulation and inflammationbioRxivSeptember 3, 2023Jian Cui, Hua Li, Guoying Zhang, Yan Zhang, Ling Yang, Martha M.S. Sim, Jeremy P. Wood, Yinan Wei, Zhenyu Li, Congqing Wuviability of BMDMs was determined using CellTiter-Glo Luminescent Cell Viability Assay (Promega, Cat#G7572). Luminescence was recorded as an indicator of ATP concentrations in metabolically active cells. BMDMs

(20) PIP5K1C phosphoinositide kinase deficiency distinguishes PIKFYVE-dependent cancer cells from non-malignant cellsAutophagySeptember 2, 2023Ajit Roy, Arup R. Chakraborty, Tyzoon Nomanbhoy, Melvin L. DePamphilisrelative to the vehicle control using the CellTiter-Glo luminescent cell viability assay (Promega, G7572) according to the manufacturer’s instructions. The IC50 values for WX8 were determined for 11 different

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(1)

1/28/2020Affordable and Highly Sensitive Reagent Mallesh KurakulaCost-effective. Easy to reconstitute. It can be stored at 4C for reuse.
(1)

8/22/2017CellTiter Glo Luminescent Cell Viability Assay for Cytotoxicity Studies Ab GCellTiter Glo is a great assay to determine cytotoxicity of drugs/agents. Reagents are resistant to many compounds that may interfere with such reagents of other cell viability assays.
(1)

2/2/2017Great Assay For Quick Cell Viability Check At Higher Throughput Tara VanderweydeThis was a great kit to use if you are screening for stressors or drugs to effect cell viability. It is quick and easy and you just need a plate reader!

CellTiter-Glo Luminescent Cell Viability Assay

CellTiter-Glo Luminescent Cell Viability Assay from Promega

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Promega

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CellTiter-Glo Luminescent Cell Viability Assay from Promega

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