Fig 1: Ethanol induces cytotoxicity on glioblastoma cells. (A) The activity of dead cell protease level which is associated with cell death was determined by CytoTox-Glo cytotoxicity assay (Promega Corp., G9292, WI, U.S.A.). Effects of ethanol on cell viability of primary glioblastoma cells (GBL-13 and GBL-15) and glioblastoma cell lines (U87MG and U373MG) are shown. This experiment was performed at 24 h, 48 h, and 72 h of ethanol treatment. (B) The LDH level in the media which is related with cell death was measured by LDH assay in GBL-13, GBL-15, U87MG and U373MG. The LDH level was measured after the 24 h ethanol treatment. Values are expressed as mean and SD. *p<0.05 compared with the control group.
Fig 2: Determination of the CC50 and EC50 of compound #9 in cell cultures. (A,B) EC50 of compound #9. HEK293T cells (A) or Vero cells (B) were infected with BRBV at an MOI of 10, followed by application of compound #9 at various concentrations as indicated. At 3 days post-treatment, RT-qPCR was performed to quantify the vgc numbers in the cell culture media. Values are the means with standard deviation and are normalized to a mock group, and were obtained from three experiments, each performed in duplicate. The EC50 was calculated using GraphPad Prism software. (C,D) CC50 of compound #9. A cytotoxicity assay was used to measure the viability of HEK293T cells (C) or Vero cells (D) affected by compound #9 at various concentrations as indicated. The percentage of viable cells were determined using a CytoTox-Glo cytotoxicity assay kit (Promega). The results are shown as relative values to the mock control cells. Values are the means with standard deviation obtained from three experiments performed in duplicate. The CC50 was calculated using GraphPad Prism software.
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