Fig 1: Influence of nAChR ligands on the release of IL-10 from murine bone marrow granulocytes. Cells were incubated in a medium containing 10 ng/ml lipopolysaccharide from E. coli without or with nicotine or a-conotoxins, as indicated. IL-10 concentrations were measured in supernatants after 23 h of cell incubation using a mouse IL-10 ELISA kit (ab108870, Abcam, United Kingdom). The average values ± SEM of 9–12 independent measurements, each performed in duplicates, are shown. The Kruskal-Wallis One Way Analysis of Variance on Ranks and the Mann-Whitney Rank Sum Test were used. ND, not detectable; *p < 0.05 compared to the cells treated with LPS only.
Supplier Page from Abcam for Mouse IL-10 ELISA Kit