Fig 1: Influence of nAChR ligands on the release of IL-10 from murine bone marrow granulocytes. Cells were incubated in a medium containing 10 ng/ml lipopolysaccharide from E. coli without or with nicotine or α-conotoxins, as indicated. IL-10 concentrations were measured in supernatants after 23 h of cell incubation using a mouse IL-10 ELISA kit (ab108870, Abcam, United Kingdom). The average values ± SEM of 9–12 independent measurements, each performed in duplicates, are shown. The Kruskal-Wallis One Way Analysis of Variance on Ranks and the Mann-Whitney Rank Sum Test were used. ND, not detectable; *p < 0.05 compared to the cells treated with LPS only.
Fig 2: Depletion of Ccl2 in microglia alters pain-associated cytokines and factors in spine ELISA for known cytokines and factors related to pain. (A) Il1β. (B) TNFα. (C) IFNɣ. (D) Il6. (E) Il17. (F) ARG1. (G) CD163. (H) TGFβ1. (I) Il10. *p<0.05. ns, non-significant.
Supplier Page from Abcam for Mouse IL-10 ELISA Kit