Fig 2: Differential STAT3 BRs. (A) STAT3 BRs divided by occupancy pattern. (B) Examples of differentially occupied STAT3 BRs. JAK3 shows strong binding in the ABC cell lines: SU-DHL2, OCI-Ly3, OCI-Ly10, and U-2932. EIF3B shows strong binding in the GCB cell lines: SU-DHL4, SU-DHL6, SU-DHL10, and OCI-Ly7.

Fig 3: Western blot analysis of STAT3 in ABC and GCB cell lines. Western immunoblots were performed on whole-cell lysate from DLBCL cell lines probed with antibodies against STAT3, tyrosine-phosphorylated STAT3, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Fold change was measured by densitometric quanitification using NIH ImageJ software, normalized to a GAPDH loading control.

Fig 4: STAT3 binding correlated with gene expression change. Red dots indicate STAT3 BRs that have significant differences in binding (FDR < 0.05) between subtypes and are also associated with genes that display significant differences in expression (FDR < 0.05); dark gray dots indicate STAT3 BRs that either show significant differences in binding or whose associated genes display a significant difference in expression; and light gray dots indicate other BRs.

Fig 5: STAT3 peaks associated with gene expression changes. Examples of differentially occupied STAT3 BRs are shown. Blue tracks represent gene expression; orange tracks represent STAT3 binding; pink bars show noteworthy STAT3 BRs. CXCR5 shows increased gene expression correlated with STAT3 binding (chr11: 118,754,259−118,754,737) in the ABC cell lines: SU-DHL2, OCI-Ly3, OCI-Ly10, and U-2932. ABI3 shows strong increased gene expression correlated with STAT3 binding (chr17: 47,287,858−47,288,168) in the GCB cell lines: SU-DHL4, SU-DHL6, SU-DHL10, and OCI-Ly7. CD22 shows increased gene expression in the GCB cell lines, but a mixture of STAT3 binding behavior: STAT3 has more GCB binding near the TSS (chr19: 35,819,821−35,820,060) but more ABC binding at two sites near the 3′ end (chr19: 35,837,564—35,837,836; chr19: 35,838,409−35,838,860).