Ubiquitin Antibody 13-1600 from Thermo Fisher Scientific

Product Specs
ItemUbiquitin Antibody
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog Number13-1600
Quantity100 µg
ApplicationsELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
FormatPBS with 0.1% sodium azide; pH 7.4
ReactivityBovine, Chicken, Human, Mouse, Xenopus laevis
TargetUbiquitin
IsotypeIgG1 kappa
CloneUbi-1
HostMouse
ImmunogenPurified bovine ubiquitin conjugated to carrier protein
ClonalityMonoclonal
Storage Temperature-20°C
Conjugate/TagUnconjugated
NCBI Full Gene Namepolyubiquitin-C-like, ribosomal protein S27a S homeolog, ubiquitin A-52 residue ribosomal protein fusion product 1, ribosomal protein S27-like, ubiquitin C, ubiquitin B, ribosomal protein S27 like, ribosomal protein S27a, ribosomal protein S27A
NCBI Gene AliasesD8Ertd21e, RPS27A, Cep52, HEL-S-50, UbI, RPL40, TI-225, S27A, Uba52, Ubb, 1810034D23Rik, UBA80, L40, 2700054O04Rik, HEL112, CEP52, Ubc, UBCEP80, polyubiquitin-C, rps27a, 0610006J14Rik, CEP80, HUBCEP52, rps27a.L, eS31, UBCEP1, Ubb2, Rps27a, UBB, UBC, Gm1863, HMG20
Antibody TypePrimary
Concentration0.5 mg/mL
Storage-20°C

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Thermo Fisher Scientific

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(1) Optogenetic-mediated induction and monitoring of α-synuclein aggregation in cellular models of Parkinson’s diseaseSTAR ProtocolsDecember 1, 2023Maxime Teixeira, Razan Sheta, Walid Idi, Abid Oueslati(ICC)",Santa Cruz,Sc-28359 "Mouse, anti-ubiquitin monoclonal (Ubi-1); 1:20 (ICC)",Thermo Fisher Scientific,13-1600 "Rabbit, anti-alpha-synuclein (pS129) (EP1536Y); 1:2,000 (ICC)",Abcam,ab51253 "Rabbit, anti-mCherry

(2) Ubiquitination of the protocadherin-γA3 variable cytoplasmic domain modulates cell-cell interactionFrontiers in Cell and Developmental BiologySeptember 18, 2023Albert Ptashnik, Nicole LaMassa, Aliya Mambetalieva, Emily Schnall, Mike Bucaro, Greg R. Phillipsantibodies to GFP (Abcam Cat# ab290, RRID:AB_303395) and mouse anti-ubiquitin (Thermo Fisher Scientific Cat# 13-1600, RRID:AB_2533002). Secondary detection employed IRDye 800CW goat anti-rabbit LI-COR Biosciences Cat#

(3) Longitudinal in vivo metabolic labeling reveals tissue-specific mitochondrial proteome turnover rates and proteins selectively altered by parkin deficiencyScientific ReportsJuly 14, 2023K. L. Stauch, S. Totusek, A. J. Trease, L. D. Estrella, K. Emanuel, A. Fangmeier, H. S. FoxSigma Aldrich [HPA004829]; NdufV2 1:750, Sigma Aldrich [HPA004829]; total ubiquitin 1:500, Invitrogen [13–1600]). Membranes were washed 3 × 10 min with TBST and then incubated with appropriate near-infrared conjugate

(4) The pro-tumorigenic cytokine IL-32 has a high turnover in multiple myeloma cells due to proteolysis regulated by oxygen-sensing cysteine dioxygenase and deubiquitinating enzymesFrontiers in OncologyMay 29, 2023Martin Kastnes, Kristin Roseth Aass, Siri Anshushaug Bouma, Charlotte Årseth, Muhammad Zahoor, Mariia Yurchenko, Therese StandalUSA), anti-GAPDH (# ab8245, Abcam, Cambridge, UK), anti-HA (#H6908, Sigma-Aldrich), anti-ubiquitin (# 13-1600, Thermo Fisher), and anti-ADO (ab134102, Abcam). Confocal imaging JJN-3 cells were seeded in poly-l-lysine

(5) Genipin Attenuates Diabetic Cognitive Impairment by Reducing Lipid Accumulation and Promoting Mitochondrial Fusion via FABP4/Mfn1 Signaling in MicrogliaAntioxidants (Basel, Switzerland)December 29, 2022Liu W, Li K, Zheng M, He L, Chen T.(MA527726), CD206 (12-2069-42), Mfn1 (MA5-24789, PA5-117691), p-Drp1-S637 (PA5-101038) and ubiquitin (13-1600) were purchased from Thermo Fisher (Shanghai, China). FABP4 (ab92501), CD68 (ab201340), NADPH oxidase

(6) Divergent self-association properties of paralogous proteins TRIM2 and TRIM3 regulate their E3 ligase activityNature communicationsDecember 8, 2022Esposito D, Dudley-Fraser J, Garza-Garcia A, Rittinger K.against GFP (Roche, 11814460001, 1:1,000), FLAG (Merck, A8592, 1:10,000), or ubiquitin (Invitrogen, 13-1600, 1:1,000), which were detected by anti-mouse-HRP secondary antibody (Dako, P0447, 1:2,000). Blots were

(7) Evidence that the transcriptional repressor ICER is regulated via the N-end rule for ubiquitinationExperimental Cell ResearchMay 1, 2022Angelo Cirinelli, Justin Wheelan, Christopher Grieg, Carlos A. Molinaanti-ubiquitin monoclonal antibodies (Ubi-1) were purchased from Invitrogen (Catalog No. 71–5500 and 13–1600) and used as directed for WB and Immunocytochemistry (ICC). Co-immunoprecipitation was performed using

(8) Arginase II protein regulates Parkin-dependent p32 degradation that contributes to Ca2+-dependent eNOS activation in endothelial cellsCardiovascular researchMarch 25, 2022Koo BH, Won MH, Kim YM, Ryoo S.(Burlington, MA, USA). PTEN-induced Kinase 1 (PINK1, Cat. No. #PA5-13402) and ubiquitin (Cat. No. #13-1600) antibodies were purchased from Invitrogen (Carlsbad, CA, USA). Antisera against Flag (Cat. No. sc-807),

(9) Interfering with mitochondrial dynamics sensitizes glioblastoma multiforme to temozolomide chemotherapyJournal of cellular and molecular medicineFebruary 1, 2022Wang N, Huang R, Yang K, He Y, Gao Y, Dong D.Sigma‐Aldrich. MitoTracker™ Red FM (M22425), Hoechst 33342 (H1399) and anti‐Ubiquitin WB Antibody (13–1600) were purchased from Invitrogen (Thermo Fisher Scientific, Inc.) (1:1,000). Anti‐phospho‐Ubiquitin (Ser65)

(10) Activation of the ubiquitin-proteasome system contributes to oculopharyngeal muscular dystrophy through muscle atrophyPLoS geneticsJanuary 1, 2022Ribot C, Soler C, Chartier A, Al Hayek S, Naït-Saïdi R, Barbezier N, Coux O, Simonelig M.dilutions for western blots were as follows: rabbit anti-PABPN1 [78] 1:2500; mouse anti-Ubiquitin Ubi-1 (13–1600, Life Technology) 1:1000; rat anti-myosin (MAC 147, Abcam ab51098) 1:10; mouse anti-α-tubulin (T5168,

(11) Epithelial SMYD5 Exaggerates IBD by Down-regulating Mitochondrial Functions via Post-Translational Control of PGC-1α StabilityCellular and molecular gastroenterology and hepatologyJanuary 1, 2022Hou Y, Sun X, Gheinani PT, Guan X, Sharma S, Zhou Y, Jin C, Yang Z, Naren AP, Yin J, et al.Chicago, IL). The primary antibodies used were as follows: anti-SMYD5 (ab81419; Abcam), anti-ubiquitin (13-1600; Invitrogen), anti-PHF20L1 (NBP1-79401), anti–PGC-1α (NBP1-04676; Novus), anti–COX I (sc-19998; Santa Cruz

(12) TRIP12 ubiquitination of glucocerebrosidase contributes to neurodegeneration in Parkinson's diseaseNeuronDecember 1, 2021Seo BA, Kim D, Hwang H, Kim MS, Ma SX, Kwon SH, Kweon SH, Wang H, Yoo JM, Choi S, et al.β 3 (Tuj1),BioLegend,Cat#; MMS-453P Mouse monoclonal anti-Ubiquitin,ThermoFisher Scientific,Cat#; 13–1600; RRID: AB_86560 Rabbit polyclonal anti-Ubiquitin,Dako,Cat#; Z0458; RRID: AB_2315524 "Rabbit monoclonal

(13) Suppressing TRAP1 sensitizes glioblastoma multiforme cells to temozolomideExperimental and therapeutic medicineNovember 1, 2021Wang N, Zhu P, Huang R, Sun L, Dong D, Gao Y.anti-phosphorylated-ubiquitin [serine 65 (Ser65) cat. no. ABS1513-I] from Merck KGaA, anti-ubiquitin (cat. no. 13-1600) from Thermo Fisher Scientific, Inc., anti-β actin (cat. no. 60008-1-Ig), anti-Bax (cat. no. 50599-2-Ig),

(14) GMPPA defects cause a neuromuscular disorder with α-dystroglycan hyperglycosylationThe Journal of clinical investigationMay 3, 2021Franzka P, Henze H, Jung MJ, Schüler SC, Mittag S, Biskup K, Liebmann L, Kentache T, Morales J, Martínez B, et al.

(15) The Pah-R261Q mouse reveals oxidative stress associated with amyloid-like hepatic aggregation of mutant phenylalanine hydroxylaseNature communicationsApril 6, 2021Aubi O, Prestegård KS, Jung-Kc K, Shi TS, Ying M, Grindheim AK, Scherer T, Ulvik A, McCann A, Spriet E, et al.

(16) The ubiquitin isopeptidase USP10 deubiquitinates LC3B to increase LC3B levels and autophagic activityThe Journal of biological chemistryJanuary 1, 2021Jia R, Bonifacino JS.MYC epitope (Santa Cruz Biotechnology, sc-789), Ub (for immunoblotting) (Thermo Fisher Scientific, 13-1600), Ub (for immunofluorescence) (Enzo Life Sciences, BML-PW8805-0500), HA epitope (BioLegend, 901501),

(17) SPTBN1 inhibits inflammatory responses and hepatocarcinogenesis via the stabilization of SOCS1 and downregulation of p65 in hepatocellular carcinomaTheranosticsJanuary 1, 2021Lin L, Chen S, Wang H, Gao B, Kallakury B, Bhuvaneshwar K, Cahn K, Gusev Y, Wang X, Wu Y, et al.(Santa Cruz Biotechnology); COMMD1, PIN1, PPARγ (Proteintech Group, Inc.); actin (A5316) (Millipore Sigma) and Ubiquitin (13-1600) (ThermoFisher Scientific). Secondary antibodies used are peroxidase-conjugated anti-mouse or anti-rabbit

(18) IL-1β suppression of VE-cadherin transcription underlies sepsis-induced inflammatory lung injuryThe Journal of clinical investigationJuly 1, 2020Xiong S, Hong Z, Huang LS, Tsukasaki Y, Nepal S, Di A, Zhong M, Wu W, Ye Z, Gao X, et al.ARP47447_P050), CREB (Cell Signaling, 48H2), p-CREB Ser133 (Cell Signaling, 9198), Ubiquitin (Invitrogen, 13-1600), VE-cadherin (Santa Cruz Biotechnology, sc-6458), ICAM-1 (Santa Cruz Biotechnology, sc-1511), LFA-1

(19) Genetic Selection Based on a Ste6^*C-HA-Ura3 Substrate Identifies New Cytosolic Quality Control Alleles in Saccharomyces cerevisiaeG3 (Bethesda, Md.)June 1, 2020Chan SN, Prasad R, Matsudaira P.used were mouse monoclonal anti-HA11 (BioLegend), mouse monoclonal anti-ubiquitin (Invitrogen Ubi-1 #13-1600), rabbit anti-URA3 serum (Davis Ng Lab), mouse monoclonal anti-PGK1 (Invitrogen) and rabbit anti-Kar2

(20) High-throughput retrotransposon-based genetic diversity of maize germplasm assessment and analysisMolecular Biology ReportsMarch 1, 2020Marwa Ghonaim, Ruslan Kalendar, Hoda Barakat, Nahla Elsherif, Naglaa Ashry, Alan H. Schulmanx Mo17 (Ames19097); 6—B73 (PI550473). M—Thermo Scientific GeneRuler DNA Ladder Mix (100–10,000 bp) 13 1600 Molecular Biology Reports (2020) 47:1589–1603 Fig. 3 Dendrogram of maize genotypes generated by the IRAP

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3/29/2016Detection of Ubiquitination by Western Blot A RWe performed Ubiquitination analysis in mouse whole lung homogenates by WB analysis to detect post translational modification.

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Ubiquitin Antibody from Thermo Fisher Scientific

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Ubiquitin Antibody from Thermo Fisher Scientific

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