Detection of Ubiquitination by Western Blot

Environ. Med.
University of Rochester
Postdoc

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Company:

Invitrogen Antibodies (Former Zymed)

Product Name:

Mouse anti-Ubiquilin

Catalog Number:

Ubiquilin / Dsk2 Antibody Monoclonal (Ubi-1) (13-1600)

We performed ubiquitination analysis in mouse whole lung homogenates by Western blot analysis to detect post translational modification.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Whole lung homogenate

Primary Incubation

1:1000 in 5% milk at 4 degrees over night

Blocking Agent

5% Milk

Secondary Incubation

1:10,000 Goat Anti-Rabbit IgG, (H+L), HRP conjugate

Tertiary Incubation

NA

Detection

ECL

Results Summary

We were able to detect difference in the levels of Ubiquitination between control and oxidant exposed samples.

Additional Notes

Ubiquilin / Dsk2 Antibody Monoclonal (Ubi-1) (13-1600)

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Summary

The Good

Works in mouse lung samples

The Bad

Noted none

The Bottom Line

Works for WB using lung homogenates

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