Fig 2: Transduction of PPARd-targeting shRNA abolishes the effects of GW501516 on HG-induced senescence and expression of senescence-associated proteins in ARPE-19 cells. (A,B) Cells stably expressing scrambled or PPARd-targeting shRNA were pretreated with 100 nM GW501516 (GW) for 24 h and then exposed to NG (5.6 mM) or HG (30 mM). After incubation for 48 h, SA ß-gal staining was performed (A) and SA ß-gal-positive cells were quantified in at least six fields (B). Scale bars indicate 100 µm. (C,D) Parallel immunoblotting for p53, p21, and SMP-30 (C) and densitometric quantification (D) were performed. Data are expressed as means ± S.E. (n = 3 for Western blotting and n = 6 for SA ß-gal staining). * p < 0.01, ** p < 0.05 compared with the NG-treated group. # p < 0.01, ## p < 0.05 compared with the HG-treated group.