A Reliable Antibody to Check Regucalcin Protein

pathology
upmc
research associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

Regucalcin

Catalog Number:

sc-130344

SMP30 (17) is recommended for detection of SMP30 of mouse and humanorigin by Western Blotting, it is a Mouse Monoclonal antibody to Regucalcin, it's might be one of the targets of beta-catenin.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Tissue lysis or cell lines lysis

Primary Incubation

1:1000 dilution, incubation overnight at 4 degrees

Blocking Agent

5% BSA or 5% non-fat milk

Secondary Incubation

1:5000-1:10000 dilution , 1hour at room temperature

Tertiary Incubation

NA

Detection

ECL

Results Summary

It's easy to get the target band with normal ECL reagent, and the exposure time is very short.

Additional Notes

None

Related Categories

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Summary

The Good

A good antibody

The Bad

None

The Bottom Line

The primary antibody and secondary antibody dilution could be increased based on the background, the antibody should be stored in a 4 degree fridge.

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