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Antibodies
Antibodies
Anti-Collagen IV antibody
Anti-Collagen IV antibody from Abcam
(3)
Write a review
Citations:
(679)
Supplier Page
Supplier Page from
Abcam for
Anti-Collagen IV antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-Collagen IV antibody
Company
Abcam
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
ab6586
Antibody Type
Primary
Quantity
100 UG
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Conjugate/Tag
Unconjugated
Format
Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities
Concentration
1 mg/ml
Gene Name
Col4a1
Reactivity
Human
Target
Collagen IV
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (679)
(1)
Adipose-Derived Mesenchymal Stem Cells Attenuate Immune Reactions Against Pig Decellularized Bronchi Engrafted into Rat Tracheal Defects
Organogenesis
December 31, 2023
Makoto Hisanaga, Tomoshi Tsuchiya, Hironosuke Watanabe, Koichiro Shimoyama, Mayumi Iwatake, Yukinori Tanoue, Keizaburo Maruyama, Hiroshi Yukawa, Kazuhide Sato, Yoshimi Kato,
et al.
anti-laminin (
Abcam
;
1:100
;
ab11575
), anti-fibronectin (
Abcam
;
1:100
;
ab6328
), and collagen IV (
Abcam
;
1:50
;
ab6586
). Peroxidase-conjugated IgG polyclonal antibody was used as the secondary antibody (Histofine simple
(2)
Enhancing decellularized vascular scaffolds with PVDF and PCL reinforcement: a fused deposition modeling approach
Frontiers in Cardiovascular Medicine
November 29, 2023
Kirill Yu. Klyshnikov, Maria A. Rezvova, Nikita V. Belikov, Tatiana V. Glushkova, Evgeny A. Ovcharenko
of bovine serum albumin for 1 h. Then, they were treated with antibodies against type IV collagen (
ab6586
,
Abcam
, Cambridge, UK) or antibodies against elastin (
E4013
,
Sigma-Aldrich
, St. Louis, MO, USA). Specimens
(3)
3D bioprinted multilayered cerebrovascular conduits to study cancer extravasation mechanism related with vascular geometry
Nature Communications
November 24, 2023
Wonbin Park, Jae-Seong Lee, Ge Gao, Byoung Soo Kim, Dong-Woo Cho
MA5-12023
,
Invitrogen
, USA; Anti-TuJ1 antibody,
32-2600
,
Invitrogen
, USA; Anti-Collagen IV antibody,
ab6586
,
Abcam
, UK; Anti-
MAP2
antibody,
Sigma-Aldrich
,
M4403
; Anti-
MAP2
antibody,
Abcam
,
ab32454
;
Anti-ZO-1
(4)
Vector-borne Trypanosoma brucei parasites develop in artificial human skin and persist as skin tissue forms
Nature Communications
November 23, 2023
Christian Reuter, Laura Hauf, Fabian Imdahl, Rituparno Sen, Ehsan Vafadarnejad, Philipp Fey, Tamara Finger, Nicola G. Jones, Heike Walles, Lars Barquist,
et al.
incubated overnight at 4 °C in a humidity chamber with the appropriate primary antibody (Anti-Collagen IV (
ab6586
;
Abcam
),
anti-Cytokeratin
5 (
MA5-12596
;
Thermo Fisher Scientific
),
anti-Cytokeratin
10 (M7002; DakoCytomation),
(5)
Desmoplastic stromal signatures predict patient outcomes in pancreatic ductal adenocarcinoma
Cell reports. Medicine
November 21, 2023
Mascharak S, Guo JL, Foster DS, Khan A, Davitt MF, Nguyen AT, Burcham AR, Chinta MS, Guardino NJ, Griffin M,
et al
.
N/A "Collagen I, polyclonal",
Abcam
,Cat#
ab34710
; RRID: AB_731684 "Collagen IV, polyclonal",
Abcam
,Cat#
ab6586
; RRID: AB_305584 "Fibronectin, polyclonal",
Abcam
,Cat#
ab23751
; RRID: AB_447656 "PD-1,
clone
D4W2J",Cell
(6)
A bioengineered in situ ovary (ISO) supports follicle engraftment and live-births post-chemotherapy
Journal of Tissue Engineering
November 17, 2023
Michael J Buckenmeyer, Meena Sukhwani, Aimon Iftikhar, Alexis L Nolfi, Ziyu Xian, Srujan Dadi, Zachary W Case, Sarah R Steimer, Antonio D’Amore, Kyle E Orwig,
et al.
Acid-Schiff (PAS). Antibodies specific for ECM proteins Collagen I (
Abcam
,
ab34710
) and IV (
Abcam
,
ab6586
), Fibronectin (
Abcam
,
ab23751
), and Laminin (
Abcam
,
ab11575
) were evaluated using DAB (3,3′-Diaminobenzidine)
(7)
Inhibition of TGF-β2–Induced Trabecular Meshwork Fibrosis by Pirfenidone
Translational Vision Science & Technology
November 17, 2023
Xiaofeng Zhu, Bei Zeng, Caiqing Wu, Zidong Chen, Minbin Yu, Yangfan Yang
60357" Anti-α-SMA,
1:10000
,
1:500
,"
Abcam
,
ab124964
" Anti-F-actin,
1:500
,
1:500
,"
Abcam
,
ab130935
" Anti-collagen IV,
1:1000
,
1:500
,"
Abcam
,
ab6586
" Anti-fibronectin,
1:1000
,
1:500
,"
Abcam
,
ab2413
" Primary Antibodies
Used in Western Blot
and Immunocytochemistry
(8)
ELABELA protects against diabetic kidney disease by activating high glucose-inhibited renal tubular autophagy
The Journal of Biomedical Research
November 15, 2023
Xiyin Zheng, Lulu Yin, Jing Song, Juan Chen, Wensha Gu, Min Shi, Hong Zhang
antibodies against ELA (1∶1000; Cat. #ab61637, Abcam, Cambridge, UK), collagen Ⅳ (Col-Ⅳ; 1∶1000; Cat. #
ab6586
,
Abcam
), fibronectin (FN; 1∶1000; Cat. #
SC-8422
,
Santa Cruz Biotechnology
, Santa Cruz, CA, USA), transforming
(9)
Unlocking the potential of ultra-high dose fractionated radiation for effective treatment of glioblastoma
Research Square
November 13, 2023
Xiao-Yan Lan, Lukasz Kalkowski, Cheng-Yan Chu, Anna Jablonska, Shen Li, Mihoko Kai, Yue Gao, Miroslaw Janowski, Piotr Walczak
Technology), Iba1 (
1:250
; Cat.
019-19741
,
Wako
);
CD45
(
1:250
; Cat.
ab10558
,
Abcam
), Collagen IV (
1:300
; Cat.
ab6586
,
Abcam
). Histochemical and
immunofluorescent
images were acquired with a
DMi8
inverted microscope (
Leica Microsystems
(10)
Crosstalk with lung fibroblasts shapes the growth and therapeutic response of mesothelioma cells
Cell Death & Disease
November 8, 2023
Yakinthi Chrisochoidou, Rajat Roy, Pooyeh Farahmand, Guadalupe Gonzalez, Jennifer Doig, Lukas Krasny, Ella F. Rimmer, Anne E Willis, Marion MacFarlane, Paul H. Huang,
et al.
(#
6763
),
MCP1
(CCL2) (#
7201
),
IL-17A
(#
4887
) were from
ProSci
. Antibodies against AR (
ab45089
), COL4A3 (
ab6586
),
ENG
(
ab169545
),
FAP
(
ab207178
),
IL-1A
(
ab227482
), IL-8 (
ab18672
) were from
Abcam
. Antibodies against
(11)
Elastin-like Recombinamer Hydrogels as Platforms for Breast Cancer Modeling
Biomacromolecules
October 9, 2023
Barbara Blanco-Fernandez, Arturo Ibañez-Fonseca, Doriana Orbanic, Celia Ximenes-Carballo, Soledad Perez-Amodio, Jose Carlos Rodríguez-Cabello, Elisabeth Engel
Anti-collagen I antibody (
ab34710
), anti-collagen III antibody (
ab7778
), anti-collagen IV antibody (
ab6586
), anti-fibronectin antibody (
ab2413
), and
goat
serum were purchased from
Abcam
. Collagen type I (Col1)
(12)
Extracellular Matrix Influences Gene Expression and Differentiation of Mouse Trophoblast Stem Cells
Stem Cells and Development
October 1, 2023
Bryony V. Natale, Ramie Kotadia, Katarina Gustin, Anirudha Harihara, Sarah Min, Michael J. Kreisman, Kellie M. Breen, David R.C. Natale
Abcam
); anti-αSMA,
1:200
(
ab5694
;
Abcam
); anti-Laminin,
1:200
(
ab11575
;
Abcam
); anti-Collagen,
1:200
(
ab6586
;
Abcam
); anti-Fibronectin,
1:200
(
ab23750
;
Abcam
) and anti-MCT4,
1:400
(
ab3314P
;
EMD Millipore
). Histological
(13)
Tumor microenvironment in a minipig model of spinal cord glioma
Journal of Translational Medicine
September 27, 2023
Muhibullah S. Tora, Stewart G. Neill, Yuliya Lakhina, Hemza Assed, Michelle Zhang, Purva P. Nagarajan, Thais Federici, Juanmarco Gutierrez, Kimberly B. Hoang, Yuhong Du,
et al.
Invitrogen, USA), Desmin (
MA106401
,
Invitrogen
, USA),
CD31
(MCA1746GA, Invitrogen, USA), Collagen IV (
ab6586
,
ABCAM
, USA) with Opal Detection Kit (AKOYA BIOSCIENCES, USA) and the images were taken by
Vectra Polaris
(14)
Self-organized and directed branching results in optimal coverage in developing dermal lymphatic networks
Nature communications
September 21, 2023
Uçar MC, Hannezo E, Tiilikainen E, Liaqat I, Jakobsson E, Nurmi H, Vaahtomeri K.
(
1:300
;
R&D systems
MAB2125
),
goat
anti-VEGFR3 (
1:150
; R&D
AF743
), rabbit anti-collagen IV (
1:150
;
Abcam
Ab6586
), chicken anti-GFP (
1:1000
;
Abcam
ab13970
) and rabbit anti-RFP (
1:500
;
Rockland
600-401-379
). The used
(15)
GATA1-defective immune-megakaryocytes as possible drivers of idiopathic pulmonary fibrosis
bioRxiv : the preprint server for biology
September 19, 2023
Gobbo F, Zingariello M, Verachi P, Falchi M, Arciprete F, Martelli F, Peli A, Mazzarini M, Vierstra J, Mead-Harvey C,
et al
.
Novus biologicals
, Centennial, CO, USA), Collagen III (cat no.
ab7778
,
Abcam
) or Collagen IV (cat no.
ab6586
,
Abcam
) over night at 4°C. Cell binding of the primary antibodies was visualized with a secondary antibody
(16)
MYCT1 attenuates renal fibrosis and tubular injury in diabetic kidney disease
iScience
September 15, 2023
Xin Li, Qiu-Ling Fan, Tian-Kui Ma, Cong Liu, Hang Shi, Yuan-Yuan Sun, Yue Wang, Dong-Xue Ding, Ao Tang, Yu Qin,
et al.
RRID:AB_2082037" Rabbit polyclonal anti-Collagen IV (
Western blot
:
1:2000
;
IHC
:
1:200
),"
Abcam
, USA","Cat#
ab6586
, RRID:AB_305584" Rabbit polyclonal anti-Fibronectin (
Western blot
:
1:2000
;
IHC
:
1:300
),"
Proteintech
Co
(17)
Age and obesity-driven changes in the extracellular matrix of the primary tumor and metastatic site influence tumor invasion and metastatic outgrowth
bioRxiv
August 29, 2023
Sydney J. Conner, Justinne R. Guarin, Hannah B. Borges, Kenneth J. Salhany, Diamond N. Mensah, Grace A. Hamilton, Giang H. Le, Madeleine J. Oudin
reagents, etc. Antibodies used
anti–collagen VI
(
ab199720
;
Abcam
, Cambridge, MA), and anti-collagen IV (
ab6586
;
Abcam
, Cambridge, MA). ECM substrates used were collagen VI (
ab7538
;
Abcam
, Cambridge, MA), collagen
(18)
Reduced smooth muscle-fibroblasts transformation potentially decreases intestinal wound healing and colitis-associated cancer in ageing mice
Signal Transduction and Targeted Therapy
August 9, 2023
Yi Liu, Yanhong Ji, Ruiyi Jiang, Chao Fang, Gang Shi, Lin Cheng, Yinan Zuo, Yixin Ye, Xiaolan Su, Junshu Li,
et al.
antibody to Laminin (
Abcam
, Cat#
ab11575
; RRID: AB_298179); Rabbit antibody to Collagen IV (
Abcam
, Cat#
ab6586
; RRID: AB_305584);Rabbit antibody to Vimentin (
clone
D21H3,
Cell Signaling Technology
, Cat#
5741
; RRID:
(19)
Engineering of extracellular matrix from human iPSC-mesenchymal progenitors to enhance osteogenic capacity of human bone marrow stromal cells independent of their age
Front Bioeng Biotechnol
August 2, 2023
Dominik Hanetseder, Tina Levstek, Andreas Herbert Teuschl-Woller, Julia Katharina Frank, Barbara Schaedl, Heinz Redl, Darja Marolt Presen
incubated with primary antibodies against collagen type I (cat. No.
ab34710
), collagen type IV (cat. No.
ab6586
), laminin (cat. No.
ab11575
) and fibronectin (cat. No.
ab2413
) purchased from
Abcam
(Cambridge, UK)
(20)
Pericyte dysfunction and impaired vasomotion are hallmarks of islets during the pathogenesis of type 1 diabetes
Cell Reports
August 1, 2023
Luciana Mateus Mateus Gonçalves, Mirza Muhammad Fahd Fahd Qadir, Maria Boulina, Madina Makhmutova, Elizabeth Pereira, Joana Almaça
A5228
CD31,
Millipore
,RRID: 550389 Tyrosine hydroxylase (TH),
Millipore
,RRID:
AB152
Collagen IV,
Abcam
,RRID:
AB6586
Periostin,R&D,RRID: AF2955 Insulin,Dako,RRID: IR002
Somatostatin
,
Millipore
,RRID:
MAB354
CD146,
Abcam
,RRID:
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Reviews
Write a Review
(1)
4/14/2022
Western Blot Collagen 4
Marco Pilli
Abcam antibody works well for Western blot, but our results had several unspecific bands.
(1)
1/14/2020
Good Antibody to Stain Collagen IV from Abcam
Purnima Gupta
Very good staining of collagen IV across the vasculature.
(1)
1/25/2017
A Collagen IV Antibody That Works For Drosophila
Ayla Sessions
The aim of our research is visualizing and quantifying the extracelullar matrix proteins in the Drosophila heart. Specifically we note how that change with age. There as of yet had been no anti-...
Read Review
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>
>>
Anti-Collagen IV antibody from Abcam
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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