Abcam
Anti-Collagen IV antibody
ab6586
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The aim of our research is visualizing and quantifying the extracelullar matrix proteins in the Drosophila heart. Specifically we note how that change with age. There as of yet had been no anti-collagen IV antibody that had been noted to work in Drosophila melanogaster in order to visualize their collagen IV homolog Viking. We were looking for one to use in immunofluorescence. Due to the promiscuous nature of this collagen IV antibody across many species we gave it a try in Drosophila and it worked fine.
Immunofluorescence
Drosophila heart tube
1:500, overnight, 4 degrees, in .1% PBST
1% Seablock for an hour prior to incubation
1:1000, 1 hour, room temp in .1% PBST of Donkey Anti-Rabbit alexa 647
None
LSM 710 Zeiss microscope in far red channel
Fine results. Stained Drosophila homolog to collagen IV [viking] very well when compared to viking-GFP tagged protein fluorescence side by side. Reproducible and quantifiable. This antibody can absolutely be used in Drosophila melanogaster. Fluorescent staining labeled as Vkg for Viking in Drosophila. It stains collagen IV in the fly heart very well.
10.1016/j.matbio.2016.10.008
N/A
It stains collagen IV in the fly heart very well.
In humans it tends to stain multiple collagen chains, but at least in Drosophila hearts on Collagen IV protein exists.
Works in Drosophila melanogaster.