Anti-Collagen IV antibody ab6586 from Abcam

Product Specs
ItemAnti-Collagen IV antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab6586
Antibody TypePrimary
Quantity100 UG
ClonalityPolyclonal
IsotypeIgG
HostRabbit
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Conjugate/TagUnconjugated
FormatImmunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities
Concentration1 mg/ml
Gene NameCol4a1
ReactivityHuman
TargetCollagen IV

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Adipose-Derived Mesenchymal Stem Cells Attenuate Immune Reactions Against Pig Decellularized Bronchi Engrafted into Rat Tracheal DefectsOrganogenesisDecember 31, 2023Makoto Hisanaga, Tomoshi Tsuchiya, Hironosuke Watanabe, Koichiro Shimoyama, Mayumi Iwatake, Yukinori Tanoue, Keizaburo Maruyama, Hiroshi Yukawa, Kazuhide Sato, Yoshimi Kato, et al.anti-laminin (Abcam; 1:100; ab11575), anti-fibronectin (Abcam; 1:100; ab6328), and collagen IV (Abcam; 1:50; ab6586). Peroxidase-conjugated IgG polyclonal antibody was used as the secondary antibody (Histofine simple

(2) Enhancing decellularized vascular scaffolds with PVDF and PCL reinforcement: a fused deposition modeling approachFrontiers in Cardiovascular MedicineNovember 29, 2023Kirill Yu. Klyshnikov, Maria A. Rezvova, Nikita V. Belikov, Tatiana V. Glushkova, Evgeny A. Ovcharenkoof bovine serum albumin for 1 h. Then, they were treated with antibodies against type IV collagen (ab6586, Abcam, Cambridge, UK) or antibodies against elastin (E4013, Sigma-Aldrich, St. Louis, MO, USA). Specimens

(3) 3D bioprinted multilayered cerebrovascular conduits to study cancer extravasation mechanism related with vascular geometryNature CommunicationsNovember 24, 2023Wonbin Park, Jae-Seong Lee, Ge Gao, Byoung Soo Kim, Dong-Woo ChoMA5-12023, Invitrogen, USA; Anti-TuJ1 antibody, 32-2600, Invitrogen, USA; Anti-Collagen IV antibody, ab6586, Abcam, UK; Anti-MAP2 antibody, Sigma-Aldrich, M4403; Anti-MAP2 antibody, Abcam, ab32454; Anti-ZO-1

(4) Vector-borne Trypanosoma brucei parasites develop in artificial human skin and persist as skin tissue formsNature CommunicationsNovember 23, 2023Christian Reuter, Laura Hauf, Fabian Imdahl, Rituparno Sen, Ehsan Vafadarnejad, Philipp Fey, Tamara Finger, Nicola G. Jones, Heike Walles, Lars Barquist, et al.incubated overnight at 4 °C in a humidity chamber with the appropriate primary antibody (Anti-Collagen IV (ab6586; Abcam), anti-Cytokeratin 5 (MA5-12596; Thermo Fisher Scientific), anti-Cytokeratin 10 (M7002; DakoCytomation),

(5) Desmoplastic stromal signatures predict patient outcomes in pancreatic ductal adenocarcinomaCell reports. MedicineNovember 21, 2023Mascharak S, Guo JL, Foster DS, Khan A, Davitt MF, Nguyen AT, Burcham AR, Chinta MS, Guardino NJ, Griffin M, et al.N/A "Collagen I, polyclonal",Abcam,Cat# ab34710; RRID: AB_731684 "Collagen IV, polyclonal",Abcam,Cat# ab6586; RRID: AB_305584 "Fibronectin, polyclonal",Abcam,Cat# ab23751; RRID: AB_447656 "PD-1, clone D4W2J",Cell

(6) A bioengineered in situ ovary (ISO) supports follicle engraftment and live-births post-chemotherapyJournal of Tissue EngineeringNovember 17, 2023Michael J Buckenmeyer, Meena Sukhwani, Aimon Iftikhar, Alexis L Nolfi, Ziyu Xian, Srujan Dadi, Zachary W Case, Sarah R Steimer, Antonio D’Amore, Kyle E Orwig, et al.Acid-Schiff (PAS). Antibodies specific for ECM proteins Collagen I (Abcam, ab34710) and IV (Abcam, ab6586), Fibronectin (Abcam, ab23751), and Laminin (Abcam, ab11575) were evaluated using DAB (3,3′-Diaminobenzidine)

(7) Inhibition of TGF-β2–Induced Trabecular Meshwork Fibrosis by PirfenidoneTranslational Vision Science & TechnologyNovember 17, 2023Xiaofeng Zhu, Bei Zeng, Caiqing Wu, Zidong Chen, Minbin Yu, Yangfan Yang60357" Anti-α-SMA,1:10000,1:500,"Abcam, ab124964" Anti-F-actin,1:500,1:500,"Abcam, ab130935" Anti-collagen IV,1:1000,1:500,"Abcam, ab6586" Anti-fibronectin,1:1000,1:500,"Abcam, ab2413" Primary Antibodies Used in Western Blot and Immunocytochemistry

(8) ELABELA protects against diabetic kidney disease by activating high glucose-inhibited renal tubular autophagyThe Journal of Biomedical ResearchNovember 15, 2023Xiyin Zheng, Lulu Yin, Jing Song, Juan Chen, Wensha Gu, Min Shi, Hong Zhangantibodies against ELA (1∶1000; Cat. #ab61637, Abcam, Cambridge, UK), collagen Ⅳ (Col-Ⅳ; 1∶1000; Cat. #ab6586, Abcam), fibronectin (FN; 1∶1000; Cat. #SC-8422, Santa Cruz Biotechnology, Santa Cruz, CA, USA), transforming

(9) Unlocking the potential of ultra-high dose fractionated radiation for effective treatment of glioblastomaResearch SquareNovember 13, 2023Xiao-Yan Lan, Lukasz Kalkowski, Cheng-Yan Chu, Anna Jablonska, Shen Li, Mihoko Kai, Yue Gao, Miroslaw Janowski, Piotr WalczakTechnology), Iba1 (1:250; Cat. 019-19741, Wako); CD45 (1:250; Cat. ab10558, Abcam), Collagen IV (1:300; Cat. ab6586, Abcam). Histochemical and immunofluorescent images were acquired with a DMi8 inverted microscope (Leica Microsystems

(10) Crosstalk with lung fibroblasts shapes the growth and therapeutic response of mesothelioma cellsCell Death & DiseaseNovember 8, 2023Yakinthi Chrisochoidou, Rajat Roy, Pooyeh Farahmand, Guadalupe Gonzalez, Jennifer Doig, Lukas Krasny, Ella F. Rimmer, Anne E Willis, Marion MacFarlane, Paul H. Huang, et al.(#6763), MCP1 (CCL2) (#7201), IL-17A (#4887) were from ProSci. Antibodies against AR (ab45089), COL4A3 (ab6586), ENG (ab169545), FAP (ab207178), IL-1A (ab227482), IL-8 (ab18672) were from Abcam. Antibodies against

(11) Elastin-like Recombinamer Hydrogels as Platforms for Breast Cancer ModelingBiomacromoleculesOctober 9, 2023Barbara Blanco-Fernandez, Arturo Ibañez-Fonseca, Doriana Orbanic, Celia Ximenes-Carballo, Soledad Perez-Amodio, Jose Carlos Rodríguez-Cabello, Elisabeth EngelAnti-collagen I antibody (ab34710), anti-collagen III antibody (ab7778), anti-collagen IV antibody (ab6586), anti-fibronectin antibody (ab2413), and goat serum were purchased from Abcam. Collagen type I (Col1)

(12) Extracellular Matrix Influences Gene Expression and Differentiation of Mouse Trophoblast Stem CellsStem Cells and DevelopmentOctober 1, 2023Bryony V. Natale, Ramie Kotadia, Katarina Gustin, Anirudha Harihara, Sarah Min, Michael J. Kreisman, Kellie M. Breen, David R.C. NataleAbcam); anti-αSMA, 1:200 (ab5694; Abcam); anti-Laminin, 1:200 (ab11575; Abcam); anti-Collagen, 1:200 (ab6586; Abcam); anti-Fibronectin, 1:200 (ab23750; Abcam) and anti-MCT4, 1:400 (ab3314P; EMD Millipore). Histological

(13) Tumor microenvironment in a minipig model of spinal cord gliomaJournal of Translational MedicineSeptember 27, 2023Muhibullah S. Tora, Stewart G. Neill, Yuliya Lakhina, Hemza Assed, Michelle Zhang, Purva P. Nagarajan, Thais Federici, Juanmarco Gutierrez, Kimberly B. Hoang, Yuhong Du, et al.Invitrogen, USA), Desmin (MA106401, Invitrogen, USA), CD31 (MCA1746GA, Invitrogen, USA), Collagen IV (ab6586, ABCAM, USA) with Opal Detection Kit (AKOYA BIOSCIENCES, USA) and the images were taken by Vectra Polaris

(14) Self-organized and directed branching results in optimal coverage in developing dermal lymphatic networksNature communicationsSeptember 21, 2023Uçar MC, Hannezo E, Tiilikainen E, Liaqat I, Jakobsson E, Nurmi H, Vaahtomeri K.(1:300; R&D systems MAB2125), goat anti-VEGFR3 (1:150; R&D AF743), rabbit anti-collagen IV (1:150; Abcam Ab6586), chicken anti-GFP (1:1000; Abcam ab13970) and rabbit anti-RFP (1:500; Rockland 600-401-379). The used

(15) GATA1-defective immune-megakaryocytes as possible drivers of idiopathic pulmonary fibrosisbioRxiv : the preprint server for biologySeptember 19, 2023Gobbo F, Zingariello M, Verachi P, Falchi M, Arciprete F, Martelli F, Peli A, Mazzarini M, Vierstra J, Mead-Harvey C, et al.Novus biologicals, Centennial, CO, USA), Collagen III (cat no. ab7778, Abcam) or Collagen IV (cat no. ab6586, Abcam) over night at 4°C. Cell binding of the primary antibodies was visualized with a secondary antibody

(16) MYCT1 attenuates renal fibrosis and tubular injury in diabetic kidney diseaseiScienceSeptember 15, 2023Xin Li, Qiu-Ling Fan, Tian-Kui Ma, Cong Liu, Hang Shi, Yuan-Yuan Sun, Yue Wang, Dong-Xue Ding, Ao Tang, Yu Qin, et al.RRID:AB_2082037" Rabbit polyclonal anti-Collagen IV (Western blot:1:2000; IHC:1:200),"Abcam, USA","Cat# ab6586, RRID:AB_305584" Rabbit polyclonal anti-Fibronectin (Western blot:1:2000; IHC:1:300),"Proteintech Co

(17) Age and obesity-driven changes in the extracellular matrix of the primary tumor and metastatic site influence tumor invasion and metastatic outgrowthbioRxivAugust 29, 2023Sydney J. Conner, Justinne R. Guarin, Hannah B. Borges, Kenneth J. Salhany, Diamond N. Mensah, Grace A. Hamilton, Giang H. Le, Madeleine J. Oudinreagents, etc. Antibodies used anti–collagen VI (ab199720; Abcam, Cambridge, MA), and anti-collagen IV (ab6586; Abcam, Cambridge, MA). ECM substrates used were collagen VI (ab7538; Abcam, Cambridge, MA), collagen

(18) Reduced smooth muscle-fibroblasts transformation potentially decreases intestinal wound healing and colitis-associated cancer in ageing miceSignal Transduction and Targeted TherapyAugust 9, 2023Yi Liu, Yanhong Ji, Ruiyi Jiang, Chao Fang, Gang Shi, Lin Cheng, Yinan Zuo, Yixin Ye, Xiaolan Su, Junshu Li, et al.antibody to Laminin (Abcam, Cat# ab11575; RRID: AB_298179); Rabbit antibody to Collagen IV (Abcam, Cat# ab6586; RRID: AB_305584)；Rabbit antibody to Vimentin (clone D21H3, Cell Signaling Technology, Cat# 5741; RRID:

(19) Engineering of extracellular matrix from human iPSC-mesenchymal progenitors to enhance osteogenic capacity of human bone marrow stromal cells independent of their ageFront Bioeng BiotechnolAugust 2, 2023Dominik Hanetseder, Tina Levstek, Andreas Herbert Teuschl-Woller, Julia Katharina Frank, Barbara Schaedl, Heinz Redl, Darja Marolt Presenincubated with primary antibodies against collagen type I (cat. No. ab34710), collagen type IV (cat. No. ab6586), laminin (cat. No. ab11575) and fibronectin (cat. No. ab2413) purchased from Abcam (Cambridge, UK)

(20) Pericyte dysfunction and impaired vasomotion are hallmarks of islets during the pathogenesis of type 1 diabetesCell ReportsAugust 1, 2023Luciana Mateus Mateus Gonçalves, Mirza Muhammad Fahd Fahd Qadir, Maria Boulina, Madina Makhmutova, Elizabeth Pereira, Joana AlmaçaA5228 CD31,Millipore,RRID: 550389 Tyrosine hydroxylase (TH),Millipore,RRID: AB152 Collagen IV,Abcam,RRID: AB6586 Periostin,R&D,RRID: AF2955 Insulin,Dako,RRID: IR002 Somatostatin,Millipore,RRID: MAB354 CD146,Abcam,RRID:

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(1)

4/14/2022Western Blot Collagen 4 Marco PilliAbcam antibody works well for Western blot, but our results had several unspecific bands.
(1)

1/14/2020Good Antibody to Stain Collagen IV from Abcam Purnima GuptaVery good staining of collagen IV across the vasculature.
(1)

1/25/2017A Collagen IV Antibody That Works For Drosophila Ayla SessionsThe aim of our research is visualizing and quantifying the extracelullar matrix proteins in the Drosophila heart. Specifically we note how that change with age. There as of yet had been no anti-... Read Review

Anti-Collagen IV antibody from Abcam

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Anti-Collagen IV antibody from Abcam

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