Anti-Fibrillarin antibody [38F3] - Nucleolar Marker ab4566 from Abcam

Product Specs
ItemAnti-Fibrillarin antibody [38F3] - Nucleolar Marker
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab4566
Antibody TypePrimary
Quantity250 UL
IsotypeIgG1
ClonalityMonoclonal
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Western blot
Clone38F3
HostMouse, Nude mouse
Conjugate/TagUnconjugated
FormatSterile filtered
Research AreaEpigenetics
Gene Namefbl
ReactivityHuman, Mouse, Rat
TargetFibrillarin

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Comparison of click-capable oxaliplatin and cisplatin derivatives to better understand Pt(ii)-induced nucleolar stress†RSC Chemical BiologyOctober 4, 2023Andres S. Guerrero, Paul D. O’Dowd, Hannah C. Pigg, Katelyn R. Alley, Darren M. Griffith, Victoria J. DeRoseMonoclonal Antibody (CR55T33, ThermoFisher, 2.5 μg in PBST with 1% BSA), (anti-Fibrillarin antibody ab4566 from Abcam, 1 : 500 dilution) and 1 hour in secondary antibody for NPM1 or γH2AX and an 1.5 h for fibrillarin

(2) CRISPR/Cas9-mediated knockout of the ubiquitin variant UbKEKS reveals a role in regulating nucleolar structures and compositionBiology OpenSeptember 15, 2023Julie Frion, Anna Meller, Gwendoline Marbach, Dominique Lévesque, Xavier Roucou, François-Michel Boisvertdense fibrillar component NPM1 (ABclonal #A17983, 1:150) and fibrillarin (monoclonal 38F3, Abcam #ab4566, 1:1000) primary antibodies were used for the overnight incubation at 4°C. Anti-mouse (Invitrogen #A-11003,

(3) R-loop landscapes in the developing human brain are linked to neural differentiation and cell-type specific transcriptionbioRxivJuly 22, 2023Elizabeth A. LaMarca, Atsushi Saito, Amara Plaza-Jennings, Sergio Espeso-Gil, Allyse Hellmich, Michael B. Fernando, Behnam Javidfar, Will Liao, Molly Estill, Kayla Townsley, et al.cat. no. #574778, 1:500), PSD-95 (Invitrogen, cat. no. #51–6900, 1:500), Fibrillarin (Abcam, cat. no. #ab4566, 1:500), and S9.6 (1:100, generated in house as described above). The following day, samples were washed

(4) Hepatic depletion of nucleolar protein mDEF causes excessive mitochondrial copper accumulation associated with p53 and NRF1 activationiScienceJuly 21, 2023Wei J, Wang S, Zhu H, Cui W, Gao J, Gao C, Yu B, Liu B, Chen J, Peng J.(ac026), CTR1 (A10109), NRF1 (A5547), SCO2 (A7051), TUBULIN (ac021) from ABclonal, COX1 (ab203912), FIB (ab4566) from Abcam (Cambridge, England) and p53 (Do-1) from Santa Cruz (CA, USA). Quantitative real-time PCR

(5) Heritable transcriptional defects from aberrations of nuclear architectureNatureJuly 1, 2023Papathanasiou S, Mynhier NA, Liu S, Brunette G, Stokasimov E, Jacob E, Li L, Comenho C, van Steensel B, Buenrostro JD, et al.H3T3 (Millipore, 07-424, 1:12,000); phospho H3S10 (Abcam, ab47297; 1:200); and fibrillarin (Abcam, ab4566; 1:500). Staining of Dam-methylated DNA in fixed cells was done using purified GFP-tagged m6A-Tracer

(6) Defining diurnal fluctuations in mouse choroid plexus and CSF at high molecular, spatial, and temporal resolutionNature CommunicationsJune 22, 2023Ryann M. Fame, Peter N. Kalugin, Boryana Petrova, Huixin Xu, Paul A. Soden, Frederick B. Shipley, Neil Dani, Bradford Grant, Aja Pragana, Joshua P. Head, et al.synthase (cloneD7V8B) (Cell Signaling #14309 (RRID: AB_2665545); 1:1000), mouse anti-fibrillarin (Abcam ab4566 (RRID: AB_304523); 1:250). The following secondary antibodies were used for immunostaining: Goat anti-Rabbit

(7) PARP-dependent and NAT10-independent acetylation of N4-cytidine in RNA appears in UV-damaged chromatinEpigenetics & ChromatinJune 15, 2023Alena Svobodová Kovaříková, Lenka Stixová, Aleš Kovařík, Eva BártováMA, USA), anti-phosphorylated histone H2AX (γH2AX; phospho S139) (#05-636, Merck), anti-fibrillarin (#ab4566, Abcam), anti- phospho-ATM; Ser1981 (#MAB3806-C, Merck), anti-α-tubulin (#ab80779 Abcam), and anti-NAT10

(8) Divergent patterns of meiotic double strand breaks and synapsis initiation dynamics suggest an evolutionary shift in the meiosis program between American and Australian marsupialsFrontiers in Cell and Developmental BiologyApril 25, 2023F. Javier Valero-Regalón, Mireia Solé, Pablo López-Jiménez, María Valerio-de Arana, Marta Martín-Ruiz, Roberto de la Fuente, Laia Marín-Gual, Marilyn B. Renfree, Geoff Shaw, Soledad Berríos, et al.Calbiochem, 1:50 dilution), rabbit anti-RPA2 (ab10359, Abcam, 1:50 dilution), mouse anti-fibrillarin (ab4566, Abcam; 1:50 dilution), human anti-centromere (441-10BK-50, Antibodies Incorporated, 1:50 dilution).

(9) Inflammasome-independent NLRP3 function enforces ATM activity in response to genotoxic stressLife science allianceApril 1, 2023Bodnar-Wachtel M, Huber AL, Gorry J, Hacot S, Burlet D, Gérossier L, Guey B, Goutagny N, Bartosch B, Ballot E, et al.1:2,000) and anti-NOXA (114C307, 1:1,000) from Santa Cruz; anti-ATM (#ab32420, 1:1,000), anti-fibrillarin (ab4566, 1:1,000), and anti-GAPDH (ab9484, 1:1,000) from Abcam; anti-FLAG (F7425 1:5,000) and anti-α-tubulin

(10) Henipaviruses and lyssaviruses target nucleolar treacle protein and regulate ribosomal RNA synthesisTraffic (Copenhagen, Denmark)March 1, 2023Rawlinson SM, Zhao T, Ardipradja K, Zhang Y, Veugelers PF, Harper JA, David CT, Sundaramoorthy V, Moseley GW.Cat #11003‐1‐AP; immunoblotting (IB), 1:2000; immunofluorescence (IF), 1:100), anti‐FBL (Abcam, Cat #Ab4566; IF, 1:100), anti‐GFP (Sigma‐Aldrich, Cat #11814460001 ROCHE; IB, 1:2000), anti‐RABV N (In‐house, 21

(11) Mapping nucleolus-associated chromatin interactions using nucleolus Hi-C reveals pattern of heterochromatin interactionsNature CommunicationsJanuary 21, 2023Ting Peng, Yingping Hou, Haowei Meng, Yong Cao, Xiaotian Wang, Lumeng Jia, Qing Chen, Yang Zheng, Yujie Sun, Hebing Chen, et al.antibodies, Actin (ab8226, Abcam, dilution 1:1000), Nucleolin (ab129000, Abcam, dilution 1:100), Fibrillarin (ab4566, Abcam, dilution 1:100) and POLR1E (A12700, Abclonal, dilution 1:100) overnight at 4 °C. Membranes were

(12) Psi promotes Drosophila wing growth via direct transcriptional activation of cell cycle targets and repression of growth inhibitorsDevelopmentJanuary 15, 2023Olga Zaytseva, Naomi C. Mitchell, Damien Muckle, Caroline Delandre, Zuqin Nie, Janis K. Werner, John T. Lis, Eduardo Eyras, Ross D. Hannan, David L. Levens, et al.antibody. Primary antibodies used for immunofluorescence were: mouse anti-fibrillarin (1:1000, Abcam ab4566), rabbit anti-phospho-Histone3 (1:5000, Abcam ab14955) and mouse anti-Dcp1 (1:500, Cell Signaling 9578S).

(13) RBP Image Database: A resource for the systematic characterization of the subcellular distribution properties of human RNA binding proteinsNucleic acids researchJanuary 6, 2023Benoit Bouvrette LP, Wang X, Boulais J, Kong J, Syed EU, Blue SM, Zhan L, Olson S, Stanton R, Wei X, et al.anti-Coilin (GTX11822, GeneTex); mouse anti-DCP1 (ENZSPA827D, Enzo Life Sciences); mouse anti-Fibrillarin (ab4566, Abcam); mouse anti-GM130 (610822, BD); mouse anti-KDEL (SC-100706, Santa Cruz Biotech); mouse anti-Phospho

(14) PQBP5/NOL10 maintains and anchors the nucleolus under physiological and osmotic stress conditionsNature CommunicationsJanuary 4, 2023Xiaocen Jin, Hikari Tanaka, Meihua Jin, Kyota Fujita, Hidenori Homma, Maiko Inotsume, Huang Yong, Kenichi Umeda, Noriyuki Kodera, Toshio Ando, et al.and 300 mM glycine for 60 min at RT. The samples were incubated with mouse anti-fibrillarin (1:100, #ab4566, Abcam), mouse anti-NPM1 (1:5000, #ab10530, Abcam), rat anti-PES1 antibody (1:2000, #252849, Abcam),

(15) Micro‐Topographies Induce Epigenetic Reprogramming and Quiescence in Human Mesenchymal Stem CellsAdvanced ScienceJanuary 1, 2023Steven Vermeulen, Bart Van Puyvelde, Laura Bengtsson del Barrio, Ruben Almey, Bernard K. van der Veer, Dieter Deforce, Maarten Dhaenens, Jan de BoerAbcam; ab1220), anti‐nucleophosmin antibody (1:200; Abcam; ab37659), anti‐fibrillarin (1:100; Abcam; ab4566), and anti‐nucleolin antibody (1:200; ThermoFisher; ZN004). Image Analysis Fixed samples were inverted,

(16) Nucleophagy delays aging and preserves germline immortalityNat AgingJanuary 1, 2023Papandreou ME, Konstantinidis G, Tavernarakis N.mouse anti-actin (catalog no. MAB1501; Sigma-Aldrich); 1:5,000 mouse anti-fibrillarin (catalog no. ab4566; Abcam); 1:1,000 mouse anti-fibrillarin (catalog no. NB300-269; Novus Biologicals); and 1:5,000 rabbit

(17) Transcription-coupled donor DNA expression increases homologous recombination for efficient genome editingNucleic Acids ResearchOctober 28, 2022Kaixuan Gao, Xuedi Zhang, Zhenwu Zhang, Xiangyu Wu, Yan Guo, Pengchong Fu, Angyang Sun, Ju Peng, Jie Zheng, Pengfei Yu, et al.from ABclonal Technology (WuHan, China). Anti-GFP (mouse mAb, ab1218) and anti-fibrillarin (mouse mAb, ab4566) were purchased from Abcam Technology. Immunofluorescence images were collected by using a Zeiss LSM

(18) TRIM26 Maintains Cell Survival in Response to Oxidative Stress through Regulating DNA Glycosylase StabilityInternational Journal of Molecular SciencesOctober 1, 2022Sifaddin M. R. Konis, Jonathan R. Hughes, Jason L. ParsonsRosenquist. Antibodies against TRIM26 (ab89290), NTH1 (ab70726), OGG1 (ab124741), and fibrillarin (ab4566) were from Abcam (Cambridge, UK). NEIL3 (sc-393703) and lamin a/c antibodies (sc-7292) were from Santa Cruz Biotechnology

(19) Bioinformatics and Functional Analysis of a New Nuclear Localization Sequence of the Influenza A Virus NucleoproteinCellsSeptember 22, 2022Nhan L. T. Nguyen, Nelly PantÃ©immunofluorescence microscopy, as indicated above. An antibody against fibrillarin (Abcam, Catalogue number: Ab4566) was used to detect the nucleolus at 1:100 dilution in BF for 1 h at room temperature. All samples were

(20) Ercc2/Xpd deficiency results in failure of digestive organ growth in zebrafish with elevated nucleolar stressiScienceSeptember 16, 2022Ma J, Shao X, Geng F, Liang S, Yu C, Zhang R.polyclonal anti-LC3B,Abcam,Cat#ab51520; RRID:AB_881429 Mouse monoclonal anti-Fibrillarin,Abcam,Cat# ab4566; RRID:AB_304523 Rabbit polyclonal anti-γH2A.X (Ser139),GeneTex,Cat#GTX127342; RRID:AB_2833105 Rabbit

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1/12/2017Great Antibody To Use As A Nucleolus Marker Tara VanderweydeWas using this antibody as part of a panel of nuclear substructure markers to identify where are protein of interest was localizing. This antibody works great at low concentrations for staining in ... Read Review

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Anti-Fibrillarin antibody [38F3] - Nucleolar Marker from Abcam

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Anti-Fibrillarin antibody [38F3] - Nucleolar Marker from Abcam

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